수입산 바리과(Family Serranidae) 잡종 어류(Epinephelus moara ♀×E. lanceolatus ♂)의 분자생물학적 판별Molecular Biological Species Identification of Imported Groupers (Epinephelus moara ♀×E. lanceolatus ♂)
- Other Titles
- Molecular Biological Species Identification of Imported Groupers (Epinephelus moara ♀×E. lanceolatus ♂)
- Authors
- 김용휘; 박종연; 김재훈; 방인철
- Issue Date
- 2020
- Publisher
- 한국수산과학회
- Keywords
- Epinephelus; co1; rag1; RAPD; Hybrid grouper
- Citation
- 한국수산과학회지, v.53, no.4, pp.566 - 571
- Journal Title
- 한국수산과학회지
- Volume
- 53
- Number
- 4
- Start Page
- 566
- End Page
- 571
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/3444
- ISSN
- 0374-8111
- Abstract
- To classify a presumed hybrid of imported grouper species acquired from the National Fishery Products Quality Management Service, maternal and paternal lines were identified based on partial sequencing of mitochondrial cytochrome c oxidase 1 (co1) and nuclear recombination activation gene 1 (rag1) genes. The matrilineal species was identified as Epinephleus moara by a partial (760 bp) co1 sequence. Ambiguous sequences with base pairs belonging to E. moara or E. lanceolatus were found in a total of 15 different base pairs in the partial 1,159 bp of the rag1 gene, and the patrilineal species was found to be E. lanceolatus. Therefore, all of the groupers examined in the study were identified to be hybrids of E. moara and E. lanceolatus. In addition, a fast and convenient method using random amplification of polymorphic DNA (RAPD) was established for hybrid discrimination. Hybrids between E. moara ♀ and E. lanceolatus ♂ were identified through specific bands of 387 bp and 433 bp in PRIMER 6.
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