Enterotoxigenic Bacteroides fragilis infection exacerbates tumorigenesis in AOM/DSS mouse modelopen access
- Authors
- Hwang, Soonjae; Lee, Chang Gun; Jo, Minjeong; Park, Chan Oh; Gwon, Sun-Yeong; Hwang, Samnoh; Yi, Hye Chin; Lee, So-Yeon; Eom, Yong-Bin; Karim, Baktiar; Rhee, Ki-Jong
- Issue Date
- 2020
- Publisher
- Ivyspring International Publisher
- Keywords
- ETBF; colorectal cancer; inflammation; azoxymethane; dextran sulfate sodium
- Citation
- International Journal of Medical Sciences, v.17, no.2, pp 145 - 152
- Pages
- 8
- Journal Title
- International Journal of Medical Sciences
- Volume
- 17
- Number
- 2
- Start Page
- 145
- End Page
- 152
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/3734
- DOI
- 10.7150/ijms.38371
- ISSN
- 1449-1907
- Abstract
- The azoxymethane (AOM)/dextran sulfate sodium (DSS) murine model is commonly used to study colitis-associated cancer. The human commensal bacterium, enterotoxigenic Bacteroides fragilis (ETBF) secretes the Bacteroides fragilis toxin (BFT) which is necessary and sufficient to cause colitis. We report that BALB/c mice infected with WT-ETBF and administered three cycles of AOM/DSS developed numerous, large-sized polyps predominantly in the colorectal region. In addition, AOM/DSS-treated BALB/c mice orally inoculated with wild-type nontoxigenic Bacteroides fragilis (WT-NTBF) overexpressing bft (rETBF) developed numerous polyps whereas mice infected with WT-NTBF overexpressing a biologically inactive bft (rNTBF) did not promote polyp formation. Unexpectedly, the combination of AOM+ETBF did not induce polyp formation whereas ETBF+DSS did induce polyp development in a subset of BALB/c mice. In conclusion, WT-ETBF promoted polyp development in AOM/DSS murine model with increased colitis in BALB/c mice. The model described herein provides an experimental platform for understanding ETBF-induced colonic tumorigenesis and studying colorectal cancer in wild-type mice.
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Collections - College of Medical Sciences > Department of Biomedical Laboratory Science > 1. Journal Articles
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