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Highly sensitive piezoelectric immunosensors employing signal amplification with gold nanoparticles

Authors
Kwak, JiwonLee, Soo Suk
Issue Date
1-Nov-2019
Publisher
Institute of Physics Publishing
Keywords
quartz crystal microbalance; immunoassay; prostate specific antigen; resonance frequency; gold staining enhancement; signal amplification
Citation
Nanotechnology, v.30, no.44
Journal Title
Nanotechnology
Volume
30
Number
44
URI
https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/4087
DOI
10.1088/1361-6528/ab36c9
ISSN
0957-4484
1361-6528
Abstract
We present a quartz crystal microbalance (QCM) immunosensor for highly sensitive detection of prostate-specific antigen (PSA) in a human serum immunoassay. In particular, in this study, we employed signal amplification using and enlarging gold nanoparticles. Because QCM measures the change of resonance frequency according to the mass change occurring on the sensor surface, we could quantitatively analyze PSA based on a tremendous increase in mass by sandwich immunoassay using AuNP-conjugated anti-PSA-detecting antibody enhanced with subsequent gold staining. The limit of detection of the PSA immunoassay in human serum without gold staining enhancement was 687 pg ml(-1) but was 48 pg ml(-1) with the gold staining-mediated signal amplification. That is, amplifying the signal resulted in increased sensitivity and reproducibility of immunoassay in a human serum.
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