Anti-Obesity Effect of Diphlorethohydroxycarmalol Isolated from Brown Alga Ishige okamurae in High-Fat Diet-Induced Obese Miceopen access
- Authors
- Ding, Yuling; Wang, Lei; Im, SeungTae; Hwang, Ouibo; Kim, Hyun-Soo; Kang, Min-Cheol; Lee, Seung-Hong
- Issue Date
- Nov-2019
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
- Keywords
- high-fat diet mice; Ishige okamurae; diphlorethohydroxycarmalol (DPHC); anti-obesity effect
- Citation
- Marine Drugs, v.17, no.11
- Journal Title
- Marine Drugs
- Volume
- 17
- Number
- 11
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/4100
- DOI
- 10.3390/md17110637
- ISSN
- 1660-3397
- Abstract
- Diphlorethohydroxycarmalol (DPHC) is one of the most abundant bioactive compounds in Ishige okamurae. The previous study suggested that DPHC possesses strong in vitro anti-obesity activity in 3T3-L1 cells. However, the in vivo anti-obesity effect of DPHC has not been determined. The current study explored the effect of DPHC on high-fat diet (HFD)-induced obesity in C57BL/6J mice. The results indicated that oral administration of DPHC (25 and 50 mg/kg/day for six weeks) significantly and dose-dependently reduced HFD-induced adiposity and body weight gain. DPHC not only decreased the levels of triglyceride, low-density lipoprotein cholesterol, leptin, and aspartate transaminase but also increased the level of high-density lipoprotein cholesterol in the serum of HFD mice. In addition, DPHC significantly reduced hepatic lipid accumulation by reduction of expression levels of the critical enzymes for lipogenesis including SREBP-1c, FABP4, and FAS. Furthermore, DPHC remarkably reduced the adipocyte size, as well as decreased the expression levels of key adipogenic-specific proteins and lipogenic enzymes including PPAR gamma, C/EBP alpha, SREBP-1c, FABP4, and FAS, which regulate the lipid metabolism in the epididymal adipose tissue (EAT). Further studies demonstrated that DPHC significantly stimulated the phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) in both liver and EAT. These results demonstrated that DPHC effectively prevented HFD-induced obesity and suggested that DPHC could be used as a potential therapeutic agent for attenuating obesity and obesity-related diseases.
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Collections - College of Medical Sciences > Department of Pharmaceutical Engineering > 1. Journal Articles
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