Protective Effects of Novel Antioxidant Peptide Purified from Alcalase Hydrolysate of Velvet Antler Against Oxidative Stress in Chang Liver Cells In Vitro and in a Zebrafish Model In Vivoopen access
- Authors
- Ding, Yuling; Ko, Seok-Chun; Moon, Sang-Ho; Lee, Seung-Hong
- Issue Date
- 2-Oct-2019
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
- Keywords
- velvet antler; alcalase hydrolysate; antioxidant peptide; protection ability; oxidative stress
- Citation
- International Journal of Molecular Sciences, v.20, no.20
- Journal Title
- International Journal of Molecular Sciences
- Volume
- 20
- Number
- 20
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/4146
- DOI
- 10.3390/ijms20205187
- ISSN
- 1661-6596
1422-0067
- Abstract
- Velvet antler has a long history in traditional medicine. It is also an important healthy ingredient in food as it is rich in protein. However, there has been no report about antioxidant peptides extracted from velvet antler by enzymatic hydrolysis. Thus, the objective of this study was to hydrolyze velvet antler using different commercial proteases (Acalase, Neutrase, trypsin, pepsin, and alpha-chymotrypsin). Antioxidant activities of different hydrolysates were investigated using peroxyl radical scavenging assay by electron spin resonance spectrometry. Among all enzymatic hydrolysates, Alcalase hydrolysate exhibited the highest peroxyl radical scavenging activity. Alcalase hydrolysate was then purified using ultrafiltration, gel filtration, and reverse-phase high performance liquid chromatography. The purified peptide was identified to be Trp-Asp-Val-Lys (tetrapeptide) with molecular weight of 547.29 Da by Q-TOF ESI mass spectroscopy. This purified peptide exhibited strong scavenging activity against peroxyl radical (IC50 value, 0.028 mg/mL). In addition, this tetrapeptide showed significant protection ability against AAPH-induced oxidative stress by inhibiting of reactive oxygen species (ROS) generation in Chang liver cells in vitro and in a zebrafish model in vivo. This research suggests that the tetrapeptide derived from Alcalase-proteolytic hydrolysate of velvet antler are excellent antioxidants and could be effectively applied as functional food ingredients and pharmaceuticals.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medical Sciences > Department of Pharmaceutical Engineering > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/4146)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.