Thioredoxin-interacting Protein (TXNIP) Mediates Thioredox-independent Antioxidant Mechanism in Endometrial Cancer Cells Treated With 1 alpha,25-dihydroxyvitamin D-3
- Authors
- Kim, Yesol; Kim, Yeon-Suk; Kim, Minjeong; Kim, Jun-Mo; Lee, Hae-Hyeog; Kim, Tae-Hee
- Issue Date
- Sep-2019
- Publisher
- International Institute of Anticancer Research
- Keywords
- Endometrial cancer; vitamin D-3; thioredoxin; thioredoxin-interacting protein
- Citation
- Anticancer Research, v.39, no.9, pp 4795 - 4803
- Pages
- 9
- Journal Title
- Anticancer Research
- Volume
- 39
- Number
- 9
- Start Page
- 4795
- End Page
- 4803
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/4260
- DOI
- 10.21873/anticanres.13664
- ISSN
- 0250-7005
1791-7530
- Abstract
- Background/Aim: To determine the mechanism of vitamin D-3-induced modulation of antioxidant-related factors in endometrial cancer, we investigated their role in apoptosis of human endometrial cancer cells exposed to vitamin D-3. Materials and Methods: The survival rate of human endometrial cancer cells was estimated after treatment with activated vitamin D-3. Reactive oxygen species (ROS) levels were measured using flow cytometry. The levels of VDR, Trx, TXNIP and apoptosis-related proteins were investigated using western blotting and immunocytochemistry in human tissues. Results: Treatment with D-3 induced apoptotic cell death and cell-cycle arrest by increasing ROS concentration. Vitamin D-3 inhibited proliferation of human endometrial cancer cells. It regulated intracellular ROS concentration in endometrial cancer cells via increased TXNIP expression. Conclusion: Antioxidant regulation via TXNIP is an important cell death mechanism in human endometrial cancer, and occurs via induction by vitamin D-3.
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Collections - College of Medicine > Department of Obstetrics and Gynecology > 1. Journal Articles
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