T-T Mismatch-Based Electrochemical Aptasensor for Ultratrace Level Detection of Hg2+ Using Electrochemically Reduced Graphene Oxide-Modified Electrode
DC Field | Value | Language |
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dc.contributor.author | Yu, Su Hwan | - |
dc.contributor.author | Kim, Tae Hyun | - |
dc.date.accessioned | 2021-08-11T09:43:30Z | - |
dc.date.available | 2021-08-11T09:43:30Z | - |
dc.date.issued | 2019-08 | - |
dc.identifier.issn | 1550-7033 | - |
dc.identifier.issn | 1550-7041 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/4370 | - |
dc.description.abstract | Ultratrace levels of mercury ions (Hg2+) were quantified utilizing differential pulse voltammetry (DPV) in aqueous solution. This method utilized a thymine (T)-rich, methylene blue-tagged DNA (MBD) and an electrochemically-reduced graphene oxide-modified glassy carbon electrode (ERGO-GCE). The ERGO-GCE was prepared by the direct reduction of graphene oxide (GO) solution on a glassy carbon electrode (GCE) using cyclic voltammetry. The MBD, as a sensing motif, was then coated on the surface of the ERGO-GCE through pi-pi stacking. In the presence of Hg2+, mercury-mediated coordination of the T-Hg2+ -T base pairs caused folding of the MBD, leading to a hairpin structure. This resulted in subsequent unbinding of MBD from the surface of the ERGO-GCE, which produced a change in redox current of the MB tag. DPV of the modified electrode showed that the MB signal decreased linearly with increases in Hg2+ concentrations in the range between 1 fM and 100 nM with a detection limit of 0.16 fM. This sensor also exhibited excellent selectivity for Hg2+ against interfering metal ions. In addition, the sensor could be regenerated by decoupling the T-Hg2+-T in cysteine, which unfolded the MBD. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | American Scientific Publishers | - |
dc.title | T-T Mismatch-Based Electrochemical Aptasensor for Ultratrace Level Detection of Hg2+ Using Electrochemically Reduced Graphene Oxide-Modified Electrode | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1166/jbn.2019.2811 | - |
dc.identifier.scopusid | 2-s2.0-85068496177 | - |
dc.identifier.wosid | 000473074400017 | - |
dc.identifier.bibliographicCitation | Journal of Biomedical Nanotechnology, v.15, no.8, pp 1824 - 1831 | - |
dc.citation.title | Journal of Biomedical Nanotechnology | - |
dc.citation.volume | 15 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1824 | - |
dc.citation.endPage | 1831 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | HEAVY-METAL IONS | - |
dc.subject.keywordPlus | GOLD NANOPARTICLES | - |
dc.subject.keywordPlus | MERCURY IONS | - |
dc.subject.keywordPlus | COLORIMETRIC DETECTION | - |
dc.subject.keywordPlus | DNA HYBRIDIZATION | - |
dc.subject.keywordPlus | ASCORBIC-ACID | - |
dc.subject.keywordPlus | WATER | - |
dc.subject.keywordPlus | FLUORESCENT | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | TRANSDUCTION | - |
dc.subject.keywordAuthor | Electrochemical Sensor | - |
dc.subject.keywordAuthor | Electrochemically Reduced Graphene Oxide | - |
dc.subject.keywordAuthor | Thymine-Mercury(II)-Thymine | - |
dc.subject.keywordAuthor | Mercury | - |
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