T-T Mismatch-Based Electrochemical Aptasensor for Ultratrace Level Detection of Hg2+ Using Electrochemically Reduced Graphene Oxide-Modified Electrode
- Authors
- Yu, Su Hwan; Kim, Tae Hyun
- Issue Date
- Aug-2019
- Publisher
- American Scientific Publishers
- Keywords
- Electrochemical Sensor; Electrochemically Reduced Graphene Oxide; Thymine-Mercury(II)-Thymine; Mercury
- Citation
- Journal of Biomedical Nanotechnology, v.15, no.8, pp 1824 - 1831
- Pages
- 8
- Journal Title
- Journal of Biomedical Nanotechnology
- Volume
- 15
- Number
- 8
- Start Page
- 1824
- End Page
- 1831
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/4370
- DOI
- 10.1166/jbn.2019.2811
- ISSN
- 1550-7033
1550-7041
- Abstract
- Ultratrace levels of mercury ions (Hg2+) were quantified utilizing differential pulse voltammetry (DPV) in aqueous solution. This method utilized a thymine (T)-rich, methylene blue-tagged DNA (MBD) and an electrochemically-reduced graphene oxide-modified glassy carbon electrode (ERGO-GCE). The ERGO-GCE was prepared by the direct reduction of graphene oxide (GO) solution on a glassy carbon electrode (GCE) using cyclic voltammetry. The MBD, as a sensing motif, was then coated on the surface of the ERGO-GCE through pi-pi stacking. In the presence of Hg2+, mercury-mediated coordination of the T-Hg2+ -T base pairs caused folding of the MBD, leading to a hairpin structure. This resulted in subsequent unbinding of MBD from the surface of the ERGO-GCE, which produced a change in redox current of the MB tag. DPV of the modified electrode showed that the MB signal decreased linearly with increases in Hg2+ concentrations in the range between 1 fM and 100 nM with a detection limit of 0.16 fM. This sensor also exhibited excellent selectivity for Hg2+ against interfering metal ions. In addition, the sensor could be regenerated by decoupling the T-Hg2+-T in cysteine, which unfolded the MBD.
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