MBP-FGF2-Immobilized Matrix Maintains Self-Renewal and Myogenic Differentiation Potential of Skeletal Muscle Stem Cells
- Authors
- Sah, Jay Prakash; Nguyen Thi Thu Hao; Kim, Yunhye; Eigler, Tamar; Tzahor, Eldad; Kim, Sang-Heon; Hwang, Yongsung; Yoon, Jeong Kyo
- Issue Date
- Jul-2019
- Publisher
- Korean Society for Stem Cell Research
- Keywords
- Skeletal muscle stem cell; Satellite cell; MBP-FGF2; Self-renewal; Myogenic differentiation
- Citation
- International Journal of Stem Cells, v.12, no.2, pp 360 - 366
- Pages
- 7
- Journal Title
- International Journal of Stem Cells
- Volume
- 12
- Number
- 2
- Start Page
- 360
- End Page
- 366
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/4399
- DOI
- 10.15283/ijsc18125
- ISSN
- 2005-3606
2005-5447
- Abstract
- The robust capacity of skeletal muscle stem cells (SkMSCs, or satellite cells) to regenerate into new muscles in vivo has offered promising therapeutic options for the treatment of degenerative muscle diseases. However, the practical use of SkMSCs to treat muscle diseases is limited, owing to their inability to expand in vitro under defined cultivation conditions without loss of engraftment efficiency. To develop an optimal cultivation condition for SkMSCs, we investigated the behavior of SkMSCs on synthetic maltose-binding protein (MBP)-fibro blast growth factor 2 (FGF2)-immobilized matrix in vitro. We found that the chemically well-defined, xeno-free MBP-FGF2-immobilized matrix effectively supports SkMSC growth without reducing their differentiation potential in vitro. Our data highlights the possible application of the MBP-FGF2 matrix for SkMSC expansion in vitro.
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Collections - Graduate School > Department of Integrated Biomedical Science > 1. Journal Articles
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