Electrochemical Detection of Ultratrace Lead Ion through Attaching and Detaching DNA Aptamer from Electrochemically Reduced Graphene Oxide Electrodeopen access
- Authors
- Yu, Su Hwan; Lee, Chang-Seuk; Kim, Tae Hyun
- Issue Date
- Jun-2019
- Publisher
- MDPI
- Keywords
- reduced graphene oxide; G-quadruplex; lead ion; electrochemical aptasensor
- Citation
- Nanomaterials, v.9, no.6
- Journal Title
- Nanomaterials
- Volume
- 9
- Number
- 6
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/4479
- DOI
- 10.3390/nano9060817
- ISSN
- 2079-4991
- Abstract
- This paper describes a simple strategy for the ultratrace level detection of Pb2+ ion based on G-quadruplex DNA and an electrochemically reduced graphene oxide (ERGO) electrode. First, ERGO was formed on a glassy carbon electrode (GCE) by the reduction of graphene oxide (GO) using cyclic voltammetry. Subsequently, a methylene blue (MB)-tagged, guanine-rich DNA aptamer (Apt) was attached to the surface of ERGO via pi-pi interaction, leading to the Apt-modified ERGO electrode. The presence of Pb2+ could generate the folding of Apt to a G-quadruplex structure. The formation of G-quadruplex resulted in detaching the Apt from the ERGO/GCE, leading to a change in redox current of the MB tag. Electrochemical measurements showed the proposed sensor had an exceptional sensitivity for Pb2+ with a linear range from 10(-15) to 10(-9) M and a detection limit of 0.51 fM. The sensor also exhibited high selectivity for Pb2+, as well as many other advantages, such as stability, reproducibility, regeneration, as well as simple fabrication and operation processes.
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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