BILATERAL OVARIO-HYSTERECTOMY INDUCED OSTEOPOROTIC RABBIT MODEL
- Authors
- Jin, E. S.; Kim, J. Y.; Min, J. K.; Jeon, S. R.; Choi, K. H.; Lee, M. S.; Jeong, J. H.
- Issue Date
- Mar-2019
- Publisher
- Wichtig Publishing
- Keywords
- animal model; micro-computed tomography; osteoporosis; ovariectomy; rabbit
- Citation
- Journal of Biological Regulators and Homeostatic Agents, v.33, no.2, pp 391 - 396
- Pages
- 6
- Journal Title
- Journal of Biological Regulators and Homeostatic Agents
- Volume
- 33
- Number
- 2
- Start Page
- 391
- End Page
- 396
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/4670
- ISSN
- 0393-974X
1724-6083
- Abstract
- Rabbit models have been proposed for the study of postmenopausal osteoporosis by bilateral ovariectomy with reduced dietary calcium intake or glucocorticoid administration. However, restricting dietary calcium intake or administering a glucocorticoid can cause secondary osteoporosis and is not representative of a pure postmenopausal osteoporosis model. The aim of this study was to establish an experimental rabbit model of osteoporosis induced by ovario-hysterectomy alone. Fourteen female New Zealand rabbits were separated into two groups of a sham (control) group and an ovario-hysterectomyinduced osteoporosis group. Tibiae were extracted 24 weeks after ovario-hysterectomy and were scanned by micro-computed tomography. The evaluation parameters were bone mineral density (BMD), trabecular bone volume (BV/TV), trabecular number (Tb.N), trabecular thickness, and trabecular separation (Tb.Sp). The tibial samples were evaluated after hematoxylin and eosin and Masson's trichrome staining. The sham group had significantly higher BMD, BV/TV, and Tb.N values and the lowest Tb.Sp value compared to the ovario-hysterectomy group. The histological analyses revealed a loss of the bony trabeculae and an increase in osteoporotic changes in the bone of the ovario-hysterectomy-induced osteoporosis group compared to the control group. Our results indicate that an ovario-hysterectomy-induced rabbit model would be a safe, reproducible model for postmenopausal osteoporosis studies.
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Collections - College of Medicine > Department of Neurosurgery > 1. Journal Articles
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