Detailed Information

Cited 0 time in webofscience Cited 4 time in scopus
Metadata Downloads

The retinal pigment epithelial response after retinal laser photocoagulation in diabetic mice

Authors
Jang, Sun YoungCho, In HwanYang, Jin YoungPark, Ha YanWoo, Sang EarnMadrakhimov, Sanjar BatirovichChang, Hun SooLyu, JungmookPark, Tae Kwann
Issue Date
Feb-2019
Publisher
Springer Verlag
Keywords
Diabetic mouse; Laser photocoagulation; Retinal pigment epithelium
Citation
Lasers in Medical Science, v.34, no.1, pp 179 - 190
Pages
12
Journal Title
Lasers in Medical Science
Volume
34
Number
1
Start Page
179
End Page
190
URI
https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/4771
DOI
10.1007/s10103-018-2680-9
ISSN
0268-8921
1435-604X
Abstract
To investigate the characteristics of regenerated retinal pigment epithelial (RPE) cells after retinal laser photocoagulation in diabetic mice. C57BL/6J mice were used to induce diabetes using intraperitoneal injection of streptozotocin. The proliferation of RPE cells after laser photocoagulation was determined using the 5-ethynyl-2-deoxyuridine (EdU) assay in both diabetic and wild-type mice. The morphological changes of RPE cells were evaluated by using Voronoi diagram from immunostaining for ss-catenin. Characteristics of regenerated cells were evaluated by quantifying the mRNA and protein levels of RPE and epithelial-mesenchymal transition (EMT) markers. There were significantly less EdU-positive cells in laser-treated areas in diabetic mice than wild-type mice. Hexagonality was extensively lost in diabetic mice. Many EdU-positive cells were co-localized with Otx2-positive cells in the center of the laser-treated areas in wild-type mice, but only EdU-positive cells were widely distributed in diabetic mice. Quantitative analysis of mRNA and protein levels showed that the expression levels of RPE markers, Pax6, Mitf, and Otx2, were significantly decreased in RPE of diabetic mice compared with that of wild-type mice, whereas the expression levels of EMT markers, vimentin and fibronectin, were significantly increased. The proliferation and hexagonality of regenerating RPE cells were impaired after laser photocoagulation, and the regenerated RPE cells lost their original properties in diabetic mice. Further clinical research is needed to elucidate the RPE response after laser photocoagulation in diabetic patients.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Medicine > Department of Ophthalmology > 1. Journal Articles
College of Medicine > Soonchunhyang Institute of Medicine > 1. Journal Articles
College of Medicine > Department of Ophthalmology > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Chang, Hun Soo photo

Chang, Hun Soo
College of Medicine (Department of Microbiology)
Read more

Altmetrics

Total Views & Downloads

BROWSE