Chlorin e6 and halogen light as a sebostatic photomedicine modulates linoleic acid-induced lipogenesis

Abstract

BackgroundsChlorin e6-mediated photodynamic therapy (Ce6-PDT) showed anti-microbial and anti-inflammatory effects on acne vulgaris model previously. Excess sebum production in sebocytes is a crucial cause of acne. However, information on the effect of Ce6-PDT associated with lipogenesis in sebocytes has not been reported so far.MethodsThe oil red O staining, triglyceride assay and cholesterol assay were used to investigate lipid synthesis in human sebocytes. The expressions of various molecular signals associated with lipogenesis were examined by western blot analysis.ResultsCe6-PDT reduced the lipid content of cultured sebocytes. The inactivation of Akt, mTOR, Raptor, PPAR and C/EBP, as well as the activation of AMPK, were revealed to be the molecular signals associated with sebostatic modulation by Ce6-PDT.ConclusionCe6-PDT using halogen light exerts antilipogenesis in sebocytes and may be utilized for the treatment of acne vulgaris.