MiR-9 Controls Chemotactic Activity of Cord Blood CD34(+) Cells by Repressing CXCR4 Expression
- Authors
- Ha, Tae Won; Kang, Hyun Soo; Kim, Tae-Hee; Kwon, Ji Hyun; Kim, Hyun Kvu; Ryu, Aeli; Jeon, Hyeji; Han, Jaeseok; Broxmeyer, Hal E.; Hwang, Yongsung; Lee, Yun Kyung; Lee, Man Ryul
- Issue Date
- Nov-2018
- Publisher
- Korean Society for Stem Cell Research
- Keywords
- microTRNA; CXCR4; Cord blood; Cell migration
- Citation
- International Journal of Stem Cells, v.11, no.2, pp 187 - 195
- Pages
- 9
- Journal Title
- International Journal of Stem Cells
- Volume
- 11
- Number
- 2
- Start Page
- 187
- End Page
- 195
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/5518
- DOI
- 10.15283/ijsc18057
- ISSN
- 2005-3606
2005-5447
- Abstract
- Improved approaches for promoting umbilical cord blood (CB) hematopoietic stem cell (HSC) homing are clinically important to enhance engraftment of CB-HSCs. Clinical transplantation of CB-HSCs is used to treat a wide range of disorders. however, an improved understanding of HSC chemotaxis is needed for facilitation of the engraftment process. We found that ectopic overexpression of miR-9 and antisense-miR-9 respectively down- and up-regulated C-X-C chemokine receptor type 4 (CXCR4) expression in CB-CD34(+) cells as well as in 293T and TF-1 cell lines. Since CXCR4 is a specific receptor for the stromal cell derived factor-1 (SDF-1) chemotactic factor, we investigated whether sense miR-9 and antisense miR-9 influenced CXCR4-mediated chemotactic mobility of primary CB-CD34(+) cells and TF-1 cells. Ectopic overexpression of sense miR-9 and antisense miR-9 respectively down- and up-regulated SDF-1-mediated chemotactic cell mobility. To our knowledge, this study is the first to report that miR-9 may play a role in regulating CXCR4 expression and SDF-1-mediated chemotactic activity of CB CD34(+) cells.
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- Appears in
Collections - Graduate School > Department of Integrated Biomedical Science > 1. Journal Articles
- College of Medicine > Department of Obstetrics and Gynecology > 1. Journal Articles
- College of Medicine > Department of Obstetrics and Gynecology > 1. Journal Articles
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