A pivotal role of AMPK signaling in medicarpin-mediated formation of brown and beige
- Authors
- Imran, Khan Mohammad; Yoon, Dahyeon; Kim, Yong-Sik
- Issue Date
- Mar-2018
- Publisher
- International Union of Biochemistry by IRL Press
- Keywords
- medicarpin; C3H10T1; 2; UCP1; AMPK; browning
- Citation
- BioFactors, v.44, no.2, pp 168 - 179
- Pages
- 12
- Journal Title
- BioFactors
- Volume
- 44
- Number
- 2
- Start Page
- 168
- End Page
- 179
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/6168
- DOI
- 10.1002/biof.1392
- ISSN
- 0951-6433
1872-8081
- Abstract
- Obesity poses a substantial threat of a worldwide epidemic and requires better understanding of adipose-tissue biology as well as necessitates research into the etiology and therapeutic interventions. In this study, Medicarpin (Med), a natural pterocarpan, was selected (by screening) as a small-molecule inducer of adipocyte differentiation among 854 candidates by using C3H10T1/2 mesenchymal stem cell; a cellular model of adipogenesis. Med induced the expression of brown-adipocyte commitment marker Bmp7 as well as the early regulators of brown fat fate Ppar, Prdm16, and Pgc-1 during differentiation of C3H10T1/2 mesenchymal stem cells. Med also induced the expression of a key thermogenic markeruncoupling protein 1 (UCP1)along with expression of other brown-fat-specific markers and beige-fat-specific markers. Of note, Med significantly reduced the expression of white fat markers too. Furthermore, Med treatment promoted formation of multilocular lipid droplets (LDs), expression of mitochondrial-biogenesis-related genes, and increased oxygen consumption. Gene silencing study revealed that Med promotes the development of brown- and beige-adipocyte characteristics in C3H10T1/2 mesenchymal stem cells through activation of the AMPK pathway, and our data allow us to propose Med as a candidate for therapeutics against obesity or related metabolic disorders. (c) 2017 BioFactors, 44(2):168-179, 2018
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