Neuronal injury and tumor necrosis factor-alpha immunoreactivity in the rat hippocampus in the early period of asphyxia-induced cardiac arrest under normothermiaopen access
- Authors
- Tae, Hyun-Jin; Kang, Il Jun; Lee, Tae-Kyeong; Cho, Jeong Hwi; Lee, Jae-Chul; Shin, Myoung Cheol; Kim, Yoon Sung; Cho, Jun Hwi; Kim, Jong-Dai; Ahn, Ji Hyeon; Park, Joon Ha; Kim, In-Shik; Lee, Hyang-Ah; Kim, Yang Hee; Won, Moo-Ho; Lee, Young Joo
- Issue Date
- Dec-2017
- Publisher
- Neural Regeneration Research
- Keywords
- nerve regeneration; post-cardiac arrest syndrome; normothermia; neuronal damage; gliosis; tumor necrosis factor-alpha; neural regeneration
- Citation
- Neural Regeneration Research, v.12, no.12, pp 2007 - 2013
- Pages
- 7
- Journal Title
- Neural Regeneration Research
- Volume
- 12
- Number
- 12
- Start Page
- 2007
- End Page
- 2013
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/6992
- DOI
- 10.4103/1673-5374.221157
- ISSN
- 1673-5374
1876-7958
- Abstract
- Low survival rate occurs in patients who initially experience a spontaneous return of circulation after cardiac arrest (CA). In this study, we induced asphyxial CA in adult male Sprague-Daley rats, maintained their body temperature at 37 +/- 0.5 degrees C, and then observed the survival rate during the post-resuscitation phase. We examined neuronal damage in the hippocampus using cresyl violet (CV) and Fluore-Jade B (F-J B) staining, and pro-inflammatory response using ionized calcium-binding adapter molecule 1 (Iba1), glial fibrillary acidic protein (GFAP), and tumor necrosis factor-alpha (TNF-alpha) immunohistochemistry in the hippocampus after asphyxial CA in rats under normothermia. Our results show that the survival rate decreased gradually post-CA (about 63% at 6 hours, 37% at 1 day, and 8% at 2 days post-CA). Rats were sacrificed at these points in time post-CA, and no neuronal damage was found in the hippocampus until 1 day post-CA. However, some neurons in the stratum pyramidale of the CA region in the hippocampus were dead 2 days post-CA. Iba-1 immunoreactive microglia in the CA1 region did not change until 1 day post-CA, and they were activated (enlarged cell bodies with short and thicken processes) in all layers 2 days post-CA. Meanwhile, GFAP-immunoreactive astrocytes did not change significantly until 2 days post-CA. TNF-alpha immunoreactivity decreased significantly in neurons of the stratum pyramidale in the CA1 region 6 hours post-CA, decreased gradually until 1 day post-CA, and increased significantly again 2 days post-CA. These findings suggest that low survival rate of normothermic rats in the early period of asphyxia-induced CA is related to increased TNF-alpha immunoreactivity, but not to neuronal damage in the hippocampal CA1 region.
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Collections - College of Medicine > Department of Emergency Medicine > 1. Journal Articles
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