In Vitro Effect of DFC-2 on Mycolic Acid Biosynthesis in Mycobacterium tuberculosis
- Authors
- Kim, Sukyung; Seo, Hoonhee; Al Mahmud, Hafij; Islam, Md Imtiazul; Kim, Yong-Sik; Lyu, Jiwon; Nam, Kung-Woo; Lee, Byung-Eui; Lee, Kee-In; Song, Ho-Yeon
- Issue Date
- Nov-2017
- Publisher
- 한국미생물·생명공학회
- Keywords
- DFC-2; mycolic acid; M. tuberculosis
- Citation
- Journal of Microbiology and Biotechnology, v.27, no.11, pp 1932 - 1941
- Pages
- 10
- Journal Title
- Journal of Microbiology and Biotechnology
- Volume
- 27
- Number
- 11
- Start Page
- 1932
- End Page
- 1941
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/7071
- DOI
- 10.4014/jmb.1705.05013
- ISSN
- 1017-7825
1738-8872
- Abstract
- DFC-2, a methyl 5-[2-(dimethylamino)ethoxy]-7,12-dioxo-7,12-dihydrodinaphtho[1,2-b:2',3'-d] furan-6-carboxylate, is reported to have antitubercular effects against Mycobacterium tuberculosis. At concentrations ranging from 0.19 to 0.39 mu g/ml, DFC-2 inhibited both drug-susceptible and -resistant strains of M. tuberculosis. Microarray analyses were employed to gain insights into the molecular mechanisms of DFC-2's action in M. tuberculosis. The most affected functional gene category was "lipid biosynthesis," which is involved in mycolic acid synthesis. The decrease in transcription of genes related to mycolic acid synthesis was confirmed by RT-PCR. Furthermore, we found that DFC-2 triggered a reduction in mycolic acid levels, showing a similar pattern to that of mycolic acid synthesis inhibitor isoniazid. These results may explain how this compound kills mycobacteria efficiently by inhibiting mycolic acid synthesis.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Natural Sciences > Department of Biology > 1. Journal Articles
- College of Medicine > Department of Internal Medicine > 1. Journal Articles
- College of Medicine > Department of Microbiology > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/7071)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.