Bone morphogenetic protein-2 immobilization on porous PCL-BCP-Col composite scaffolds for bone tissue engineering
DC Field | Value | Language |
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dc.contributor.author | Song, Myeong-Jin | - |
dc.contributor.author | Amirian, Jhaleh | - |
dc.contributor.author | Nguyen Thuy Ba Linh | - |
dc.contributor.author | Lee, Byong-Taek | - |
dc.date.accessioned | 2021-08-11T14:24:28Z | - |
dc.date.available | 2021-08-11T14:24:28Z | - |
dc.date.issued | 2017-09-05 | - |
dc.identifier.issn | 0021-8995 | - |
dc.identifier.issn | 1097-4628 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/7220 | - |
dc.description.abstract | The objective of this study was to develop novel porous composite scaffolds for bone tissue engineering through surface modification of polycaprolactone-biphasic calcium phosphate-based composites (PCL-BCP). PCL-BCP composites were first fabricated with salt-leaching method followed by aminolysis. Layer by layer (LBL) technique was then used to immobilize collagen (Col) and bone morphogenetic protein (BMP-2) on PCL-BCP scaffolds to develop PCL-BCP-Col-BMP-2 composite scaffold. The morphology of the composite was examined by scanning electron microscopy (SEM). The efficiency of grafting of Col and BMP-2 on composite scaffold was measured by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). Both XPS and FTIR confirmed that Col and BMP-2 were successfully immobilized into PCL-BCP composites. MC3TC3-E1 preosteoblasts cells were cultivated on composites to determine the effect of Col and BMP-2 immobilization on cell viability and proliferation. PCL-BCP-Col-BMP-2 showed more cell attachment, cell viability, and proliferation bone factors compared to PCL-BCP-Col composites. In addition, in vivo bone formation study using rat models showed that PCL-BCP-Col-BMP-2 composites had better bone formation than PCL-BCP-Col scaffold in critical size defect with 4 weeks of duration. These results suggest that PCL-BCP-Col-BMP-2 composites can enhance bone regeneration in critical size defect in a rat model with 4 weeks of duration. (C) 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45186. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | John Wiley & Sons Inc. | - |
dc.title | Bone morphogenetic protein-2 immobilization on porous PCL-BCP-Col composite scaffolds for bone tissue engineering | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1002/app.45186 | - |
dc.identifier.scopusid | 2-s2.0-85018967053 | - |
dc.identifier.wosid | 000402201600019 | - |
dc.identifier.bibliographicCitation | Journal of Applied Polymer Science, v.134, no.33 | - |
dc.citation.title | Journal of Applied Polymer Science | - |
dc.citation.volume | 134 | - |
dc.citation.number | 33 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.subject.keywordPlus | MESOPOROUS BIOACTIVE GLASS | - |
dc.subject.keywordPlus | SURFACE MODIFICATION | - |
dc.subject.keywordPlus | REGENERATION | - |
dc.subject.keywordPlus | POLYCAPROLACTONE | - |
dc.subject.keywordPlus | BIOMATERIAL | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | NANO | - |
dc.subject.keywordAuthor | composite | - |
dc.subject.keywordAuthor | in vivo | - |
dc.subject.keywordAuthor | LBL technique | - |
dc.subject.keywordAuthor | PCL-BCP-Col-BMP-2 | - |
dc.subject.keywordAuthor | surface modification | - |
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