Bone morphogenetic protein-2 immobilization on porous PCL-BCP-Col composite scaffolds for bone tissue engineering
- Authors
- Song, Myeong-Jin; Amirian, Jhaleh; Nguyen Thuy Ba Linh; Lee, Byong-Taek
- Issue Date
- 5-Sep-2017
- Publisher
- John Wiley & Sons Inc.
- Keywords
- composite; in vivo; LBL technique; PCL-BCP-Col-BMP-2; surface modification
- Citation
- Journal of Applied Polymer Science, v.134, no.33
- Journal Title
- Journal of Applied Polymer Science
- Volume
- 134
- Number
- 33
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/7220
- DOI
- 10.1002/app.45186
- ISSN
- 0021-8995
1097-4628
- Abstract
- The objective of this study was to develop novel porous composite scaffolds for bone tissue engineering through surface modification of polycaprolactone-biphasic calcium phosphate-based composites (PCL-BCP). PCL-BCP composites were first fabricated with salt-leaching method followed by aminolysis. Layer by layer (LBL) technique was then used to immobilize collagen (Col) and bone morphogenetic protein (BMP-2) on PCL-BCP scaffolds to develop PCL-BCP-Col-BMP-2 composite scaffold. The morphology of the composite was examined by scanning electron microscopy (SEM). The efficiency of grafting of Col and BMP-2 on composite scaffold was measured by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). Both XPS and FTIR confirmed that Col and BMP-2 were successfully immobilized into PCL-BCP composites. MC3TC3-E1 preosteoblasts cells were cultivated on composites to determine the effect of Col and BMP-2 immobilization on cell viability and proliferation. PCL-BCP-Col-BMP-2 showed more cell attachment, cell viability, and proliferation bone factors compared to PCL-BCP-Col composites. In addition, in vivo bone formation study using rat models showed that PCL-BCP-Col-BMP-2 composites had better bone formation than PCL-BCP-Col scaffold in critical size defect with 4 weeks of duration. These results suggest that PCL-BCP-Col-BMP-2 composites can enhance bone regeneration in critical size defect in a rat model with 4 weeks of duration. (C) 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45186.
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Collections - College of Medicine > Department of Regenerative Medicine > 1. Journal Articles
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