Identification of CEA-interacting proteins in colon cancer cells and their changes in expression after irradiation
DC Field | Value | Language |
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dc.contributor.author | Yoo, Byong Chul | - |
dc.contributor.author | Yeo, Seung-Gu | - |
dc.date.accessioned | 2021-08-11T14:24:32Z | - |
dc.date.available | 2021-08-11T14:24:32Z | - |
dc.date.issued | 2017-09 | - |
dc.identifier.issn | 2234-1900 | - |
dc.identifier.issn | 2234-3156 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/7249 | - |
dc.description.abstract | Purpose: The serum carcinoembryonic antigen (CEA) level has been recognized as a prognostic factor in colorectal cancer, and associated with response of rectal cancer to radiotherapy. This study aimed to identify CEA-interacting proteins in colon cancer cells and observe post-irradiation changes in their expression. Materials and Methods: CEA expression in colon cancer cells was examined by Western blot analysis. Using an antiCEA antibody or IgG as a negative control, immunoprecipitation was performed in colon cancer cell lysates. CEA and IgG immunoprecipitates were used for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Proteins identified in the CEA immunoprecipitates but not in the IgG immunoprecipitates were selected as CEA-interacting proteins. After radiation treatment, changes in expression of CEA-interacting proteins were monitored by Western blot analysis. Results: CEA expression was higher in SNU-81 cells compared with LoVo cells. The membrane localization of CEA limited the immunoprecipitation results and thus the number of CEA-interacting proteins identified. Only the Ras-related protein Rab-6B and lysozyme C were identified as CEA-interacting proteins in LoVo and SNU-81 cells, respectively. Lysozyme C was detected only in SNU-81, and CEA expression was differently regulated in two cell lines; it was down-regulated in LoVo but up-regulated in SNU-81 in radiation dosage-dependent manner. Conclusion: CEA-mediated radiation response appears to vary, depending on the characteristics of individual cancer cells. The lysozyme C and Rab subfamily proteins may play a role in the link between CEA and tumor response to radiation, although further studies are needed to clarify functional roles of the identified proteins. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Korean Society for Therapeutic Radiology and Oncology | - |
dc.title | Identification of CEA-interacting proteins in colon cancer cells and their changes in expression after irradiation | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.3857/roj.2017.00255 | - |
dc.identifier.scopusid | 2-s2.0-85032839293 | - |
dc.identifier.wosid | 000413844600012 | - |
dc.identifier.bibliographicCitation | Radiation Oncology Journal, v.35, no.3, pp 281 - 288 | - |
dc.citation.title | Radiation Oncology Journal | - |
dc.citation.volume | 35 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 281 | - |
dc.citation.endPage | 288 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002267250 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | esci | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | CARCINOEMBRYONIC ANTIGEN | - |
dc.subject.keywordPlus | PREOPERATIVE CHEMORADIOTHERAPY | - |
dc.subject.keywordPlus | DOWN-REGULATION | - |
dc.subject.keywordPlus | BREAST-CANCER | - |
dc.subject.keywordPlus | RECTAL-CANCER | - |
dc.subject.keywordPlus | LYSOZYME | - |
dc.subject.keywordPlus | METASTASIS | - |
dc.subject.keywordPlus | FAMILY | - |
dc.subject.keywordPlus | BETA | - |
dc.subject.keywordPlus | CHEMOTHERAPY | - |
dc.subject.keywordAuthor | Carcinoembryonic antigen | - |
dc.subject.keywordAuthor | Colorectal neoplasms | - |
dc.subject.keywordAuthor | Rab-6B | - |
dc.subject.keywordAuthor | Lysozyme C | - |
dc.subject.keywordAuthor | Radiation | - |
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