Identification of CEA-interacting proteins in colon cancer cells and their changes in expression after irradiation
- Authors
- Yoo, Byong Chul; Yeo, Seung-Gu
- Issue Date
- Sep-2017
- Publisher
- Korean Society for Therapeutic Radiology and Oncology
- Keywords
- Carcinoembryonic antigen; Colorectal neoplasms; Rab-6B; Lysozyme C; Radiation
- Citation
- Radiation Oncology Journal, v.35, no.3, pp 281 - 288
- Pages
- 8
- Journal Title
- Radiation Oncology Journal
- Volume
- 35
- Number
- 3
- Start Page
- 281
- End Page
- 288
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/7249
- DOI
- 10.3857/roj.2017.00255
- ISSN
- 2234-1900
2234-3156
- Abstract
- Purpose: The serum carcinoembryonic antigen (CEA) level has been recognized as a prognostic factor in colorectal cancer, and associated with response of rectal cancer to radiotherapy. This study aimed to identify CEA-interacting proteins in colon cancer cells and observe post-irradiation changes in their expression. Materials and Methods: CEA expression in colon cancer cells was examined by Western blot analysis. Using an antiCEA antibody or IgG as a negative control, immunoprecipitation was performed in colon cancer cell lysates. CEA and IgG immunoprecipitates were used for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Proteins identified in the CEA immunoprecipitates but not in the IgG immunoprecipitates were selected as CEA-interacting proteins. After radiation treatment, changes in expression of CEA-interacting proteins were monitored by Western blot analysis. Results: CEA expression was higher in SNU-81 cells compared with LoVo cells. The membrane localization of CEA limited the immunoprecipitation results and thus the number of CEA-interacting proteins identified. Only the Ras-related protein Rab-6B and lysozyme C were identified as CEA-interacting proteins in LoVo and SNU-81 cells, respectively. Lysozyme C was detected only in SNU-81, and CEA expression was differently regulated in two cell lines; it was down-regulated in LoVo but up-regulated in SNU-81 in radiation dosage-dependent manner. Conclusion: CEA-mediated radiation response appears to vary, depending on the characteristics of individual cancer cells. The lysozyme C and Rab subfamily proteins may play a role in the link between CEA and tumor response to radiation, although further studies are needed to clarify functional roles of the identified proteins.
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