Metabolome Profiling of Partial and Fully Reprogrammed Induced Pluripotent Stem Cells
- Authors
- Park, Soon-Jung; Lee, Sang A.; Prasain, Nutan; Bae, Daekyeong; Kang, Hyunsu; Ha, Taewon; Kim, Jong Soo; Hong, Ki-Sung; Mantel, Charlie; Moon, Sung-Hwan; Broxmeyer, Hal E.; Lee, Man Ryul
- Issue Date
- 15-May-2017
- Publisher
- Mary Ann Liebert Inc.
- Keywords
- metabolism; oxidative phosphorylation; reprogramming efficiency; metabolomics
- Citation
- Stem Cells and Development, v.26, no.10, pp 734 - 742
- Pages
- 9
- Journal Title
- Stem Cells and Development
- Volume
- 26
- Number
- 10
- Start Page
- 734
- End Page
- 742
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/7558
- DOI
- 10.1089/scd.2016.0320
- ISSN
- 1547-3287
1557-8534
- Abstract
- Acquisition of proper metabolomic fate is required to convert somatic cells toward fully reprogrammed pluripotent stem cells. The majority of induced pluripotent stem cells (iPSCs) are partially reprogrammed and have a transcriptome different from that of the pluripotent stem cells. The metabolomic profile and mitochondrial metabolic functions required to achieve full reprogramming of somatic cells to iPSC status have not yet been elucidated. Clarification of the metabolites underlying reprogramming mechanisms should enable further optimization to enhance the efficiency of obtaining fully reprogrammed iPSCs. In this study, we characterized the metabolites of human fully reprogrammed iPSCs, partially reprogrammed iPSCs, and embryonic stem cells (ESCs). Using capillary electrophoresis time-of-flight mass spectrometry-based metabolomics, we found that 89% of analyzed metabolites were similarly expressed in fully reprogrammed iPSCs and human ESCs (hESCs), whereas partially reprogrammed iPSCs shared only 74% similarly expressed metabolites with hESCs. Metabolomic profiling analysis suggested that converting mitochondrial respiration to glycolytic flux is critical for reprogramming of somatic cells into fully reprogrammed iPSCs. This characterization of metabolic reprogramming in iPSCs may enable the development of new reprogramming parameters for enhancing the generation of fully reprogrammed human iPSCs.
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Collections - Graduate School > Department of Integrated Biomedical Science > 1. Journal Articles
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