시판 쌈장에서 분리한 용혈성 Bacillus cereus의 동정 및 특성 조사Identification and Characterization of Hemolytic Bacillus cereus Isolated from Commercial Ssam-jang
- Other Titles
- Identification and Characterization of Hemolytic Bacillus cereus Isolated from Commercial Ssam-jang
- Authors
- 김동민; 박상국; 오계헌
- Issue Date
- 2017
- Publisher
- 한국생물공학회
- Keywords
- Ssam-jang; Hemolysis; Sodium chloride; Bacillus cereus MH-2
- Citation
- Korean Society for Biotechnology and Bioengineering Journal, v.32, no.3, pp 179 - 186
- Pages
- 8
- Journal Title
- Korean Society for Biotechnology and Bioengineering Journal
- Volume
- 32
- Number
- 3
- Start Page
- 179
- End Page
- 186
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/8088
- DOI
- 10.7841/ksbbj.2017.32.3.179
- ISSN
- 1225-7117
2288-8268
- Abstract
- This study was undertaken to identify and characterize hemolytic Bacillus cereus isolated from commercial ssam-jang. The physiological and biochemical properties of isolate were first examined. Using the BIOLOG system, the isolate was identified and assigned to B. cereus MH-2. Phylogenetic tree of MH-2 was plotted based on 16S rRNA sequence comparisons. Hemolytic activity was observed around wells of sheep blood agar plates seeded with MH-2 cultures; the zone of hemolysis gradually increased with increasing incubation time of the cultures. Zymographic analysis estimated the molecular weight of the presumed hemolysis-causing molecule to be about 30 kDa. Survival rates of MH-2 cells decreased with increasing NaCl concentrations in the media.
The stress shock proteins (e.g., DnaK and GroEL) induced by NaCl were reduced in proportion to the NaCl concentration and exposure period to B. cereus MH-2. Analysis of SDSPAGE and Western blot revealed that the stress shock proteins, 70-kDa DnaK and 60-kDa GroEL were decreased proportionate to the NaCl concentrations as well as exposure period in exponentially growing cultures. Scanning electron microscopy demonstrated the presence of perforations and irregular rod forms with wrinkled surfaces in cells treated with NaCl.
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