Determination of human serum albumin using a single-walled carbon nanotube-FET modified with bromocresol green
- Authors
- Kim, Bongsu; Kim, Tae Hyun
- Issue Date
- Apr-2016
- Publisher
- Springer Verlag
- Keywords
- Nanosensor; Field effect transistor; Self-assembled monolayer; Photolithography; Conductivity sensor; Amperometry
- Citation
- Microchimica Acta, v.183, no.4, pp 1513 - 1518
- Pages
- 6
- Journal Title
- Microchimica Acta
- Volume
- 183
- Number
- 4
- Start Page
- 1513
- End Page
- 1518
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/9236
- DOI
- 10.1007/s00604-016-1815-6
- ISSN
- 0026-3672
1436-5073
- Abstract
- We developed a nanoscale-based assay for the detection of human serum albumin (HSA) by placing the HSA indicator probe, bromocresol green (BCG) on the single-walled carbon nanotubes on a field effect transistor (swCNT-FET) via simple coating. Bovine serum albumin (BSA) was used as a blocking agent to reduce non-specific binding. The selective binding of HSA onto BCG alters the charge of the dye and the surface, and this causes a change in the conductance of the swCNT-FET. The assay is highly sensitive and selective for HSA, with an 18.6 nga <...mL(-1) detection limit. The high sensitivity and selectivity of the assay is ascribed to appropriate BSA blocking and the instantaneous response of sensitive swCNT-FET to rapid BCG-HSA binding, which minimizes non-specific binding and overcomes the time-sensitivity of BCG-HSA binding, respectively.
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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