PH-Sensitive Twin Liposomes Containing Quercetin and Laccase for Tumor Therapy
- Authors
- Kim, Y.[Kim, Y.]; Oh, K.T.[Oh, K.T.]; Youn, Y.S.[Youn, Y.S.]; Lee, E.S.[Lee, E.S.]
- Issue Date
- 17-Aug-2022
- Publisher
- American Chemical Society
- Citation
- Biomacromolecules, v.23, no.9, pp.3688 - 3697
- Indexed
- SCIE
SCOPUS
- Journal Title
- Biomacromolecules
- Volume
- 23
- Number
- 9
- Start Page
- 3688
- End Page
- 3697
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/100589
- DOI
- 10.1021/acs.biomac.2c00571
- ISSN
- 1525-7797
- Abstract
- In this study, functional twin liposomes (TLs) were designed by linking avidin-anchored single liposomes and biotin-anchored single liposomes via avidin-biotin interactions. Here, we first punched a hole on the liposome surface using the liposome magnetoporation method to prepare functional single liposomes, which were used for safely encapsulating quercetin (QER, as a model prodrug) or laccase (LAC, as a bioactive enzyme) inside the liposomes without the use of organic solvents; the pores were then plugged by pH-sensitive glycol chitosan grafted with 3-diethylaminopropylamine (GDEAP) and avidin (or biotin). As a result, single liposomes with QER and biotin-GDEAP were efficiently coupled with other liposomes with LAC and avidin-GDEAP. We demonstrated that the TLs could accelerate QER and LAC release at acidic pH (6.8), improving the LAC-mediated oxidization of QER and significantly elevating tumor cell death, suggesting that this strategy can be used as an efficient method for the programmed action of prodrugs. © 2022 American Chemical Society. All rights reserved.
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Collections - Pharmacy > Department of Pharmacy > 1. Journal Articles
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