Polymyxin B/chlorine e6 conjugated hyaluronate dot particles for antimicrobial photodynamic therapy
- Authors
- Kim, Y.[Kim, Y.]; Oh, K.T.[Oh, K.T.]; Youn, Y.S.[Youn, Y.S.]; Lee, E.S.[Lee, E.S.]
- Issue Date
- May-2023
- Publisher
- John Wiley and Sons Ltd
- Keywords
- antimicrobial photodynamic therapy; chlorine e6; gram-negative bacteria; hyaluronate dot; polymyxin B
- Citation
- Polymers for Advanced Technologies, v.34, no.5, pp.1557 - 1564
- Indexed
- SCIE
SCOPUS
- Journal Title
- Polymers for Advanced Technologies
- Volume
- 34
- Number
- 5
- Start Page
- 1557
- End Page
- 1564
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/103373
- DOI
- 10.1002/pat.5988
- ISSN
- 1042-7147
- Abstract
- In this study, we report ultrafine-sized antibiotic dot particles targeting gram-negative bacteria. The water-soluble ultrafine-sized hyaluronate dot (dHA) was conjugated with polymyxin B (PMB, as a target material for lipopolysaccharide on a gram-negative bacteria) and chlorine e6 (Ce6, as a model photosensitizer), and it was denoted as dHA-(PMB/Ce6). These dot particles interacted with lipopolysaccharide (LPS) on gram-negative bacteria, which enabled PMB-mediated docking to the outer membrane of gram-negative cells. In the antimicrobial experiments using Escherichia coli cells, the phototoxic singlet oxygen generated from the dHA-(PMB/Ce6) under light irradiation inhibited E. coli cell growth. This demonstrated that dHA-(PMB/Ce6) can provide light-activated selective phototoxicity against gram-negative bacteria. We expect that the dot particle system can be used as an efficient method for improved antimicrobial treatment. © 2023 John Wiley & Sons Ltd.
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- Appears in
Collections - Pharmacy > Department of Pharmacy > 1. Journal Articles
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