Intracellular Dynamics-Resolved Label-Free Scattering Reveals Real-Time Metabolism of Single Bacteria
- Authors
- Kim, J[Kim, Jungwoo]; Ahn, SB[Ahn, Soo Bin]; Hong, SB[Hong, Subin]; Kim, KS[Kim, Kwang-sun]; Ko, EHE[Ko, Esther Ha-eun]; Jo, IJ[Jo, I. Jeong]; Chang, JA[Chang, JuOae]; Kim, M[Kim, Meehyein]; Lee, WS[Lee, Wonsik]; Lee, HM[Lee, Haemi]
- Issue Date
- 31-Aug-2023
- Publisher
- AMER CHEMICAL SOC
- Keywords
- label-free single-bacterial cell imaging; Rayleigh scattering; metabolic status; persister subpopulation; antibiotic resistance
- Citation
- NANO LETTERS, v.23, no.17, pp.8225 - 8232
- Indexed
- SCIE
SCOPUS
- Journal Title
- NANO LETTERS
- Volume
- 23
- Number
- 17
- Start Page
- 8225
- End Page
- 8232
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/108499
- DOI
- 10.1021/acs.nanolett.3c02370
- ISSN
- 1530-6984
- Abstract
- Nanoscopic investigation of bacterial cells is essential to reveal their physiological status, impacting all cellular functions. Currently, this requires labeled probes or targeted staining procedures. Herein, we report a new bacterial feature, intracellular dynamics-resolved Rayleigh scattering (IDRS), that visualizes spatiotemporal cytoplasmic transitions in unlabeled bacteria and characterizes their real-time physiological status in 10 s. From single-bacterium IDRS signals, we discovered unique spatial patterns and their multiple transitions in Gram-negative and Gram-positive bacteria. The magnitude of IDRS signal variation highly correlated with the metabolic status of bacteria, differentiating persistent subpopulations. This is also the first report demonstrating distinct real-time metabolic conditions of unlabeled drug-resistant bacteria that are exposed to different doses of antibiotics. Our strategy opens up a way to simultaneously trace in situ metabolic and antibiotic resistance statuses, which can be applied in single-cell level control of bacterial metabolism and efficacy with a heterogeneous nature.
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Collections - Pharmacy > Department of Pharmacy > 1. Journal Articles
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