Plasmonic Annular Nanotrenches with 1 nm Nanogaps for Detection of SARS-CoV-2 Using SERS-Based Immunoassay
- Authors
- Lee, Sungwoo; Lee, Seonghyeon; Park, Woongkyu; Lee, Soohyun; Kwon, Sunwoo; Oh, Myeong Jin; Haddadnezhad, Mohammadnavid; Jung, Insub; Kim, Bohyeon; Park, Jieun; Shin, Kyu Sung; Lee, Hyungdon; Yoo, Junsang; Kim, Won-Keun; Park, Sungho
- Issue Date
- 1-Apr-2024
- Publisher
- AMER CHEMICAL SOC
- Keywords
- Au nanoparticle; nanogap; SERS; SARS-CoV-2; immunoassay
- Citation
- NANO LETTERS, v.24, no.14, pp 4233 - 4240
- Pages
- 8
- Indexed
- SCIE
SCOPUS
- Journal Title
- NANO LETTERS
- Volume
- 24
- Number
- 14
- Start Page
- 4233
- End Page
- 4240
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/110410
- DOI
- 10.1021/acs.nanolett.4c00451
- ISSN
- 1530-6984
1530-6992
- Abstract
- This study represents the synthesis of a novel class of nanoparticles denoted as annular Au nanotrenches (AANTs). AANTs are engineered to possess embedded, narrow circular nanogaps with dimensions of approximately 1 nm, facilitating near-field focusing for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) via a surface-enhanced Raman scattering (SERS)-based immunoassay. Notably, AANTs exhibited an exceedingly low limit of detection (LOD) of 1 fg/mL for SARS-CoV-2 spike glycoproteins, surpassing the commercially available enzyme-linked immunosorbent assay (ELISA) by 6 orders of magnitude (1 ng/mL from ELISA). To assess the real-world applicability, a study was conducted on 50 clinical samples using an SERS-based immunoassay with AANTs. The results revealed a sensitivity of 96% and a selectivity of 100%, demonstrating the significantly enhanced sensing capabilities of the proposed approach in comparison to ELISA and commercial lateral flow assay kits.
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