Gold nanocluster-loaded hybrid albumin nanoparticles with fluorescence-based optical visualization and photothermal conversion for tumor detection/ablation
- Authors
- Park, S.[Park, S.]; Kim, H.[Kim, H.]; Lim, S.C.[Lim, S.C.]; Lim, K.[Lim, K.]; Lee, E.S.[Lee, E.S.]; Oh, K.T.[Oh, K.T.]; Choi, H.-G.[Choi, H.-G.]; Youn, Y.S.[Youn, Y.S.]
- Issue Date
- 28-Jun-2019
- Publisher
- Elsevier B.V.
- Keywords
- Albumin nanoparticles; Antitumor therapy; FRET; Gold nanoclusters; Hyperthermia; LSPR
- Citation
- Journal of Controlled Release, v.304, pp.7 - 18
- Indexed
- SCIE
SCOPUS
- Journal Title
- Journal of Controlled Release
- Volume
- 304
- Start Page
- 7
- End Page
- 18
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/15453
- DOI
- 10.1016/j.jconrel.2019.04.036
- ISSN
- 0168-3659
- Abstract
- Gold nanoclusters (AuNCs) are viewed as effective hyperthermal agents for the treatment of tumors. Whereas AuNCs formed by the agglomeration of several to tens of gold atoms (<1–2 nm) possess significant fluorescence, they have a negligible hyperthermal effect, while AuNCs comprised of spherical gold nanoparticles (AuNPs > a few nanometers) have a marked hyperthermic effect but lose their inherent fluorescence and obstruct the intensity of neighboring fluorescent dyes due to Forster resonance energy transfer (FRET). To achieve both hyperthermia and fluorescence-based optical visualization, we generated hybrid albumin nanoparticles containing AuNCs (~88 nm) comprising AuNPs (~4.5 nm). We generated a series of formulated AuNCs and optimized the size, morphology, NIR absorbance (600–900 nm), hyperthermal activity, and fluorescence spectral characters of the resulting hybrid albumin nanoparticles (AuNCs/BSA-NPs) by considering the interparticle distance between the AuNPs and Cy5.5. Among these, AuNCs/BSA-NPs (formula D) had a strong hyperthermic effect and had well-preserved fluorescence intensity (from the attached Cy5.5) due to localized surface plasmon resonance (LSPR) and a reduction in FRET. These AuNCs/BSA-NPs were able to elevate the surface tumor temperature of HCT116-bearing mice to >50 °C following 808 nm laser irradiation (1.5 W/cm 2 , 10 min), which remarkably suppressed tumor growth (17.8 ± 16.9 mm 3 vs. PBS and AuNCs/BSA-NPs (formula E): ~1850 and ~1250 mm 3 , respectively). Also, Cy5.5-modified AuNCs/BSA-NPs (formula D) showed good performance in optical fluorescence imaging of target tumors in HCT116 tumor-bearing mice. Together, our results indicate that the interparticle distance between albumin or Cy5.5 and AuNPs/AuNCs can be optimized to achieve both hyperthermia and fluorescence emission by striking a balance between LSPR and FRET effects. We believe that the AuNC/BSA-NPs formulation presented here can serve as a potential platform for both optically visualizing and treating colon cancers. © 2019 Elsevier B.V.
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