Search for bacterial α1,2-fucosyltransferases for whole-cell biosynthesis of 2′-fucosyllactose in recombinant Escherichia coli
- Authors
- Seydametova, E.[Seydametova, E.]; Yu, J.[Yu, J.]; Shin, J.[Shin, J.]; Park, Y.[Park, Y.]; Kim, C.[Kim, C.]; Kim, H.[Kim, H.]; Yu, S.H.[Yu, S.H.]; Park, Y.[Park, Y.]; Kweon, D.-H.[Kweon, D.-H.]
- Issue Date
- 2019
- Publisher
- Elsevier GmbH
- Keywords
- Escherichia coli; Fucosyllactose; Fucosyltransferase; Human milk oligosaccharides
- Citation
- Microbiological Research, v.222, pp.35 - 42
- Indexed
- SCIE
SCOPUS
- Journal Title
- Microbiological Research
- Volume
- 222
- Start Page
- 35
- End Page
- 42
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/15768
- DOI
- 10.1016/j.micres.2019.02.009
- ISSN
- 0944-5013
- Abstract
- 2′-Fucosyllactose (2′-FL) is the most abundant human milk oligosaccharide and is important for infant nutrition and health. Because 2′-FL has potential as a functional ingredient in advanced infant formula and as a prebiotic in various foods, a cost-effective method for 2′-FL production is desirable. α1,2-Fucosyltransferase (α1,2-FT) is one of the key enzymes enabling the microbial biosynthesis of this complex sugar. However, the α1,2-FTs reported so far for the whole-cell biosynthesis of 2′-FL originate from pathogens, posing a potential hurdle for approval as a food production method depending on countries. In this study, 10 α1,2-FT genes from bacteria of biosafety level one were identified, and the main features of the deduced amino acid sequences were characterized. Four codon-optimized α1,2-FT genes were synthesized and introduced into Escherichia coli ΔL M15 strain containing the plasmid pBCGW encoding guanosine 5′-diphosphate-L-fucose biosynthetic enzymes. Among the four genes, 2′-FL was produced only by the α1,2-FT from Thermosynechococcus elongatus (Te2FT). Bifidobacterium thermacidophilum α1,2-FT (Bt2FT) showed high expression but was not active in E. coli ΔL M15. The other two α1,2-FTs were not expressed to a detectable level. During batch flask fermentation of Te2FT-expressing E. coli ΔL M15 cells, 0.49 g/L 2′-FL was obtained after 72 h of induction. This is comparable to the values previously reported for α1,2-FTs from Helicobacter pylori and Bacteroides fragilis. © 2019 Elsevier GmbH
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Collections - Biotechnology and Bioengineering > Integrative Biotechnology > 1. Journal Articles
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