X-ray as Irradiation Alternative for K562 Feeder Cell Inactivation in Human Natural Killer Cell Expansion
- Authors
- Kim, GH[Kim, Gwang Ho]; Dang, HN[Hoang-Nguyen Dang]; Phan, MTT[Minh-Trang Thi Phan]; Kweon, SH[Kweon, Soon Ho]; Chun, S[Chun, Sejong]; Cho, D[Cho, Duck]
- Issue Date
- Oct-2018
- Publisher
- INT INST ANTICANCER RESEARCH
- Keywords
- X-ray; irradiation; K562; feeder cell; inactivation
- Citation
- ANTICANCER RESEARCH, v.38, no.10, pp.5767 - 5772
- Indexed
- SCIE
SCOPUS
- Journal Title
- ANTICANCER RESEARCH
- Volume
- 38
- Number
- 10
- Start Page
- 5767
- End Page
- 5772
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/18331
- DOI
- 10.21873/anticanres.12915
- ISSN
- 0250-7005
- Abstract
- Background/Aim: gamma-Irradiation has been proven to be the most effective method to inactivate K562 cells, but gamma-irradiators are not available in some institutes. This study was designed to compare the effects of X-ray and gamma-irradiation on K562 cells in natural killer (NK) cell expansion. Materials and Methods: To expand NK cells, isolated peripheral blood mononuclear cells (PBMCs) were co-cultured with gamma-irradiated or X-ray-treated K562 cells plus IL-2 and IL-15. Characteristics of expanded NK cells were identified by flow cytometry. Results: NK cell expansion rate tended be to lower in the X-ray-treated group (68.9 +/- 32.6) than the gamma-irradiated group (78 +/- 28.7), but the difference was not significant (p=0.39). Furthermore, NK cell functions or receptor expression were similar in the two groups. Conclusion: Our results suggest that X-ray treatment can be used as an alternative to gamma-irradiation for K562 cells inactivation in human NK cell expansion.
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- Appears in
Collections - Medicine > Department of Medicine > 1. Journal Articles
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