Innovative Cryopreservation Process Using a Modified Core/Shell Cell-Printing with a Microfluidic System for Cell-Laden Scaffolds
- Authors
- Lee, JY[Lee, Jae Yoon]; Koo, Y[Koo, YoungWon]; Kim, G[Kim, GeunHyung]
- Issue Date
- 21-Mar-2018
- Publisher
- AMER CHEMICAL SOC
- Keywords
- cryopreservation; organ banking; cell-printing; cell-laden scaffold
- Citation
- ACS APPLIED MATERIALS & INTERFACES, v.10, no.11, pp.9257 - 9268
- Indexed
- SCIE
SCOPUS
- Journal Title
- ACS APPLIED MATERIALS & INTERFACES
- Volume
- 10
- Number
- 11
- Start Page
- 9257
- End Page
- 9268
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/20645
- DOI
- 10.1021/acsami.7b18360
- ISSN
- 1944-8244
- Abstract
- This work investigated the printability and applicability of a core/shell cell-printed scaffold for medium-term (for up to 20 days) cryopreservation and subsequent cultivation with acceptable cellular activities including cell viability. We developed an innovative cell-printing process supplemented with a microfluidic channel, a core/shell nozzle, and a low-temperature working stage to obtain a cell-laden 3D porous collagen scaffold for cryopreservation. The 3D porous biomedical scaffold consisted of core/shell struts with a cell laden collagen-based bioink/dimethyl sulfoxide mixture in the core region and an alginate/poly(ethylene oxide) mixture in the shell region. Following 2 weeks of cryopreservation, the cells (osteoblast-like cells or human adipose stem cells) in the scaffold showed good viability (over 90%), steady growth, and mineralization similar to those of a control scaffold fabricated using a conventional cell-printing process without cryopreservation. We believe that these results are attributable to the optimized fabrication processes the cells underwent, including safe freezing/thawing processes. On the basis of these results, this fabrication process has great potential for obtaining core/shell cell-laden collagen scaffolds for cryopreservation, which have various tissue engineering applications.
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- Appears in
Collections - Biotechnology and Bioengineering > Department of Bio-mechatronic Engineering > 1. Journal Articles
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