A Sensitive and Specific Liquid Chromatography-Tandem Mass Spectrometry Assay for Simultaneous Quantification of Salivary Melatonin and Cortisol: Development and Comparison with Immunoassays
- Authors
- Shin, S.[Shin, S.]; Oh, H.[Oh, H.]; Park, H.R.[Park, H.R.]; Joo, E.Y.[Joo, E.Y.]; Lee, S.-Y.[Lee, S.-Y.]
- Issue Date
- Jan-2021
- Publisher
- Seoul National University, Institute for Cognitive Science
- Keywords
- Cortisol; Immunoassay; Liquid chromatography-tandem mass spectrometry (LC-MS/MS); Melatonin; Performance; Saliva
- Citation
- Annals of Laboratory Medicine, v.41, no.1, pp.108 - 113
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- Annals of Laboratory Medicine
- Volume
- 41
- Number
- 1
- Start Page
- 108
- End Page
- 113
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/25412
- DOI
- 10.3343/alm.2021.41.1.108
- ISSN
- 2234-3806
- Abstract
- Melatonin and cortisol are clinically important for diagnosing sleep and mood disorders. We developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/ MS) assay for simultaneous measurement of salivary melatonin and cortisol concentrations according to the Clinical and Laboratory Standards Institute guidelines. Additionally, we compared the LC-MS/MS assay with immunoassays, ELISA (Direct Salivary Melatonin Elisa EK-DSM, Bühlmann Laboratories AG, Schönenbuch, Switzerland) and electrochemiluminescence immunoassay (Cortisol II, Roche, Mannheim, Germany), using 121 saliva samples. The LC-MS/MS assay exhibited good performance in terms of linearity, precision, accuracy, limit of detection, lower limit of quantification, extraction recovery, carry-over, and matrix effect. The LC-MS/MS assay and immunoassays showed strong correlation (Pearson’s r=0.910 for melatonin, r=0.955 for cortisol), but demonstrated a significant mean bias of 23.2% (range 54.0–143.7%) for melatonin and 48.9% (range 59.7–184.7%) for cortisol. Our LC-MS/MS assay provided more sensitive and reliable salivary melatonin and cortisol quantification results compared with immunoassays. © Korean Society for Laboratory Medicine.
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