Airborne particulate matter increases MUC5AC expression by downregulating Claudin-1 expression in human airway cellsopen access
- Authors
- Kim, S.-S.[Kim, S.-S.]; Kim, C.H.[Kim, C.H.]; Kim, J.W.[Kim, J.W.]; Kung, H.C.[Kung, H.C.]; Park, T.W.[Park, T.W.]; Shin, Y.S.[Shin, Y.S.]; Kim, J.D.[Kim, J.D.]; Ryu, S.[Ryu, S.]; Kim, W.-J.[Kim, W.-J.]; Choi, Y.H.[Choi, Y.H.]; Song, K.S.[Song, K.S.]
- Issue Date
- 2017
- Publisher
- KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
- Citation
- BMB REPORTS, v.50, no.10, pp.516 - 521
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BMB REPORTS
- Volume
- 50
- Number
- 10
- Start Page
- 516
- End Page
- 521
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/25926
- DOI
- 10.5483/BMBRep.2017.50.10.100
- ISSN
- 1976-6696
- Abstract
- CLB2.0, a constituent of PM, induces secretion of multiple cytokines and chemokines that regulate airway inflammation. Specifically, IL-6 upregulates CLB2.0-induced MUC5AC and MUC1 expression. Interestingly, of the tight junction proteins examined, claudin-1 expression was inhibited by CLB2.0 While the overexpression of claudin-1 decreased CLB2.0-induced MUC5AC expression, it increased the expression of the anti-inflammatory mucin, MUC1. CLB2.0-induced IL-6 secretion was mediated by ROS. The ROS scavenger N-acetyl-cysteine inhibited CLB2.0-induced IL-6 secretion, thereby decreasing the CLB2.0-induced MUC5AC expression, whereas CLB2.0-induced MUC1 expression increased. CLB2.0 activated the ERK1/2 MAPK via a ROS-dependent pathway. ERK1/2 downregulated the claudin-1 and MUC1 expressions, whereas it dramatically increased CLB2.0-induced MUC5AC expression. These findings suggest that CLB2.0-induced ERK1/2 activation acts as a switch for regulating inflammatory conditions though a ROS-dependent pathway. Our data also suggest that secreted IL-6 regulates CLB2.0-induced MUC5AC and MUC1 expression via ROS-mediated downregulation of claudin-1 expression to maintain mucus homeostasis in the airway.
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Collections - Medicine > Department of Medicine > 1. Journal Articles
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