CKAP2 (cytoskeleton-associated protein2) is a new prognostic marker in HER2-negative luminal type breast canceropen access
- Authors
- Sim, S.H.[Sim, S.H.]; Bae, C.-D.[Bae, C.-D.]; Kwon, Y.[ Kwon, Y.]; Hwang, H.-L.[ Hwang, H.-L.]; Poojan, S.[ Poojan, S.]; Hong, H.-I.[ Hong, H.-I.]; Kim, K.[ Kim, K.]; Kang, S.-H.[ Kang, S.-H.]; Kim, H.-S.[ Kim, H.-S.]; Um, T.-H.[ Um, T.-H.]; Park, I.H.[ Park, I.H.]; Lee, K.S.[ Lee, K.S.]; Jung, S.-Y.[ Jung, S.-Y.]; Lee, S.[ Lee, S.]; Kang, H.-S.[ Kang, H.-S.]; Lee, E.S.[ Lee, E.S.]; Kim, M.-K.[ Kim, M.-K.]; Hong, K.-M.[ Hong, K.-M.]; Ro, J.[ Ro, J.]
- Issue Date
- 3-Aug-2017
- Publisher
- PUBLIC LIBRARY SCIENCE
- Citation
- PLOS ONE, v.12, no.8
- Indexed
- SCIE
SCOPUS
- Journal Title
- PLOS ONE
- Volume
- 12
- Number
- 8
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/27892
- DOI
- 10.1371/journal.pone.0182107
- ISSN
- 1932-6203
- Abstract
- Background Recently, we reported cytoskeleton-associated protein2 (CKAP2) as a possible new prognostic breast cancer marker. However, it has not yet been applied in clinic. Therefore, clinical significance of CKAP2 was evaluated in comparison with that of Ki-67 in a cohort of breast cancer patients, and the expression difference was analyzed in cell cycle-arrested cancer and fibroblast cells. Methods A total of 579 early breast cancer patients who underwent surgery at the National Cancer Center Hospital in Korea between 2001 and 2005 were accrued. CKAP2-positive cell count (CPCC) and Ki-67 labeling index (Ki-67LI) were evaluated by immunohistochemcal staining. The immunocytochemical staining patterns of CKAP2 and Ki-67 were analyzed in HeLa and human fibroblast cells after synchronization by double thymidine block. Results Although there was a significant correlation (R = 0.754, P < 0.001) between CPCC and Ki-67LI, only CPCC was correlated with DFS in overall population (HR, 2.029; 95% CI, 1.012-4.068; P = 0.046) and HER2-negative luminal subgroup (HR, 3.984; 95% CI, 1.350-11.762; P = 0.012) by multivariate analysis. In immunocytochemical staining, more than 50% of serum-starved or non-mitotic cell phase HeLa cells were positive for Ki-67, in comparison to the low CKAP2-positivity, which might explain the prognostic difference between CPCC and Ki-67LI. Conclusions The current study showed that CPCC but not Ki-67LI is an independent prognostic indicator in early breast cancer, more specifically in HER2-negative luminal breast cancer. The difference between two markers may be related to the lower background expression of CKAP2 in cancer cells.
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Collections - Medicine > Department of Medicine > 1. Journal Articles
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