Xenotransplantation of layer-by-layer encapsulated non-human primate islets with a specified immunosuppressive drug protocol
- Authors
- Haque, M.R.[Haque, M.R.]; Kim, J.[ Kim, J.]; Park, H.[ Park, H.]; Lee, H.S.[ Lee, H.S.]; Lee, K.W.[Lee, K.W.]; Al-Hilal, T.A.[ Al-Hilal, T.A.]; Jeong, J.-H.[ Jeong, J.-H.]; Ahn, C.-H.[ Ahn, C.-H.]; Lee, D.S.[Lee, D.S.]; Kim, S.J.[Kim, S.J.]; Byun, Y.[ Byun, Y.]
- Issue Date
- 28-Jul-2017
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Layer-by-layer; Non-human primate pancreatic islets; Polyethylene glycol; Xenotransplantation
- Citation
- JOURNAL OF CONTROLLED RELEASE, v.258, pp.10 - 21
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF CONTROLLED RELEASE
- Volume
- 258
- Start Page
- 10
- End Page
- 21
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/28142
- DOI
- 10.1016/j.jconrel.2017.04.021
- ISSN
- 0168-3659
- Abstract
- Islet transplantation is as effective as but also less immunogenic than pancreas transplantation for the treatment of type 1 diabetes mellitus. However, as the complete elimination of immunogenicity still remains a major obstacle in islet transplantation, layer-by-layer encapsulation (LbL) of pancreatic islets using biocompatible polymers offers a rational approach to reducing host immune response towards transplanted islets. We investigated the effect of LbL of non-human primate (NHP) islets on reducing immunogenicity as a preclinical model since NHPs have close phylogenetic and immunological relationship with humans. LbL with three-layers of polyethylene glycol (PEG) molecules (SH-6-arm-PEG-NHS, 6-arm-PEG-catechol and linear PEG-SH) showed a uniform nano-shielding on islets without the loss of viability or function of islets. An immunosuppressive drug protocol was also combined to improve the survival rate of the transplanted islets in vivo. A xenorecipient (C57BL/6 mice) of LbL islet transplanted along with our immunosuppressive drug protocol showed 100% survival rate for 150 days after transplantation. On the other hand, naked islet recipients showed poor survival time of 5.5 +/- 1.4 days without drugs and 77.5 +/- 42 days with the drug protocol. Immunohistochemistry of the transplanted grafts and serum cytokine concentration demonstrated less immunogenicity in the LbL islet transplanted recipients compared with the naked islet ones.
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- Appears in
Collections - Engineering > Chemical Engineering > 1. Journal Articles
- Medicine > Department of Medicine > 1. Journal Articles
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