A simple and reliable analytical method based on HPLC-UV to determine oleanonic acid content in Chios gum mastic for quality control
- Authors
- Jin, Y.[Jin, Y.]; Zhao, J.[Zhao, J.]; Jeong, K.M.[Jeong, K.M.]; Yoo, D.E.[Yoo, D.E.]; Han, S.Y.[Han, S.Y.]; Choi, S.-Y.[ Choi, S.-Y.]; Ko, D.-H.[ Ko, D.-H.]; Kim, H.-J.[ Kim, H.-J.]; Sung, N.-H.[ Sung, N.-H.]; Lee, J.[Lee, J.]
- Issue Date
- Jan-2017
- Publisher
- PHARMACEUTICAL SOC KOREA
- Keywords
- Chios gum mastic; Oleanonic acid; Method validation; Quality control
- Citation
- ARCHIVES OF PHARMACAL RESEARCH, v.40, no.1, pp.49 - 56
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- ARCHIVES OF PHARMACAL RESEARCH
- Volume
- 40
- Number
- 1
- Start Page
- 49
- End Page
- 56
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/30659
- DOI
- 10.1007/s12272-016-0853-2
- ISSN
- 0253-6269
- Abstract
- A reliable analytical method based on high-performance liquid chromatography-ultraviolet detection was established for the determination of oleanonic acid (OA) content in Chios gum mastic (CGM). A simple method involving methanol extraction of CGM powder followed by basification and ether extraction was developed to isolate the triterpenic fraction including OA. The triterpenic fraction was chromatographed on a Phenomenex Gemini C-18 column (150 x 4.6 mm, 5 A mu m) under a simple gradient elution of a mobile phase containing acetonitrile and water at a flow rate of 1.0 mL min(-1). The detection wavelength was set at 210 nm. Good linearity was achieved in the range of 100.0-1000.0 mu g mL(-1) with r(2) > 0.9993, and the limit of quantification was 32.22 mu g mL(-1). Accuracy measured at three concentration levels was in the range of 93.72-99.56%, while intra-day and inter-day precisions estimated using both OA standard and CGM samples were no more than 2.83 and 4.57% RSD, respectively. Finally, this method was applied to real CGM samples from various batches, revealing that the OA contents were between 88.13 and 100.83 mu g mg(-1). These results suggest that the current method can be applied as an efficient analytical method for quality control of CGM.
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Collections - Pharmacy > Department of Pharmacy > 1. Journal Articles
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