Helicobacter pylori Eradication Can Reverse the Methylation-Associated Regulation of miR-200a/b in Gastric Carcinogenesis
- Authors
- Choi, JM[Choi, Ji Min]; Kim, SG[Kim, Sang Gyun]; Yang, HJ[Yang, Hyo-Joon]; Lim, JH[Lim, Joo Hyun]; Cho, NY[Cho, Nam-Yun]; Kim, WH[Kim, Woo Ho]; Kim, JS[Kim, Joo Sung]; Jung, HC[Jung, Hyun Chae]
- Issue Date
- Sep-2020
- Publisher
- EDITORIAL OFFICE GUT & LIVER
- Keywords
- Helicobacter pylori; MicroRNAs; Methylation; Epigenetic alteration; Stomach neoplasm
- Citation
- GUT AND LIVER, v.14, no.5, pp.571 - 580
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- GUT AND LIVER
- Volume
- 14
- Number
- 5
- Start Page
- 571
- End Page
- 580
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/3357
- DOI
- 10.5009/gnl19299
- ISSN
- 1976-2283
- Abstract
- Background/Aims: Epigenetic change is one of the mechanisms that regulates the expression of microRNAs (miRNAs) and is known to play a role in Helicobacter pylori-associated gastric carcinogenesis. We aimed to evaluate the epigenetic changes of miR-200a/b in H. pylori-associated gastric carcinogenesis and restoration after eradication. Methods: The expression and methylation levels of miR-200a/b were evaluated in gastric cancer (GC) cell lines, human gastric mucosa of H. pylori-negative and-positive controls, and H. pylori-positive GC patients. Next, the changes in the expression and methylation levels of miR-200a/b were compared between H. pylori-eradication and H. pylori-persistence groups at 6 months. Real-time reverse transcription-polymerase chain reaction was conducted to investigate the miRNA expression levels, and MethyLight was performed to assess the methylation levels. Results: In the GC cell lines, the level of miR200a/b methylation decreased and the level of expression increased after demethylation. In the human gastric mucosa, the miR-200a/b methylation levels increased in the following group order: H. pylori-negative control group, H. pylori-positive control group, and H. pylori-positive GC group. Conversely, the miR-200a/b expression levels decreased in the same order. In the H. pylori-persistence group, no significant changes were observed in the methylation and expression levels of miR-200a/b after 6 months, whereas the level of methylation decreased and the level of expression of miR-200a/b increased significantly 6 months in the H. pylori-eradication group. Conclusions: Epigenetic alterations of miR-200a/b may be implicated in H. pylori-induced gastric carcinogenesis. This field defect for cancerization is suggested to be improved by H. pylori eradication.
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Collections - Medicine > Department of Medicine > 1. Journal Articles
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