Critical role of protein L-isoaspartyl methyltransferase in basic fibroblast growth factor-mediated neuronal cell differentiationopen access
- Authors
- Dung, TTM[Dung, To Thi Mai]; Yi, YS[Yi, Young-Su]; Heo, J[Heo, Jieun]; Yang, WS[Yang, Woo Seok]; Kim, JH[Kim, Ji Hye]; Kim, HG[Kim, Han Gyung]; Park, JG[Park, Jae Gwang]; Yoo, BC[Yoo, Byong Chul]; Cho, JY[Cho, Jae Youl]; Hong, S[Hong, Sungyoul]
- Issue Date
- 31-Aug-2016
- Publisher
- KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
- Keywords
- Akt; Basic fibroblast growth factor; Mitogen-activated protein kinase; Neuronal differentiation; Phosphatidylinositide 3-kinase; Protein L-isoaspartyl methyltransferase
- Citation
- BMB REPORTS, v.49, no.8, pp.437 - 442
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BMB REPORTS
- Volume
- 49
- Number
- 8
- Start Page
- 437
- End Page
- 442
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/35512
- DOI
- 10.5483/BMBRep.2016.49.8.020
- ISSN
- 1976-6696
- Abstract
- We aimed to study the role of protein L-isoaspartyl methyltransferase (PIMT) in neuronal differentiation using basic fibroblast growth factor (bFGF)-induced neuronal differentiation, characterized by cell-body shrinkage, long neurite outgrowth, and expression of neuronal differentiation markers light and medium neurofilaments (NF). The bFGF-mediated neuronal differentiation of PC12 cells was induced through activation of mitogen-activated protein kinase (MAPK) signaling molecules [MAPK kinase 1/2 (MEK1/2), extracellular signal-regulated kinase 1/2 (ERK1/2), and p90RSK], and phosphatidylinositide 3-kinase (PI3K)/Akt signaling molecules PI3Kp110 beta, PI3Kp110 gamma, Akt, and mTOR. Inhibitors (adenosine dialdehyde and S-adenosylhomocysteine) of protein methylation suppressed bFGF-mediated neuronal differentiation of PC12 cells. PIMT-eficiency caused by PIMT-specific siRNA inhibited neuronal differentiation of PC12 cells by suppressing phosphorylation of MEK1/2 and ERK1/2 in the MAPK signaling pathway and Akt and mTOR in the PI3K/Akt signaling pathway. Therefore, these results suggested that PIMT was critical for bFGF-mediated neuronal differentiation of PC12 cells and regulated the MAPK and Akt signaling pathways.
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- Appears in
Collections - Biotechnology and Bioengineering > Integrative Biotechnology > 1. Journal Articles
- Biotechnology and Bioengineering > Department of Genetic Engineering > 1. Journal Articles
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