Immunomodulatory effect of captopril and local irradiation on myeloid-derived suppressor cellsopen access
- Authors
- Cho, W.K.[Cho, W.K.]; Shin, S.-W.[ Shin, S.-W.]; Kim, S.-Y.[ Kim, S.-Y.]; Hong, C.-W.[ Hong, C.-W.]; Choi, C.[ Choi, C.]; Park, W.[Park, W.]; Noh, J.M.[Noh, J.M.]
- Issue Date
- 2016
- Publisher
- Department of Radiation Oncology
- Keywords
- Angiotensin converting enzyme inhibitors; Neutrophil; Radiotherapy
- Citation
- Radiation Oncology Journal, v.34, no.3, pp.223 - 229
- Indexed
- SCOPUS
KCI
- Journal Title
- Radiation Oncology Journal
- Volume
- 34
- Number
- 3
- Start Page
- 223
- End Page
- 229
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/40487
- DOI
- 10.3857/roj.2016.01816
- ISSN
- 2234-1900
- Abstract
- Purpose: This study is to investigate the effect of captopril when combined with irradiation. Materials and Methods: 4T1 (mouse mammary carcinoma) cells were injected in the right hind leg of Balb/c mice. Mice were randomized to four groups; control (group 1), captopril-treated (group 2), irradiated (group 3), irradiated and captopril-treated concurrently (group 4). Captopril was administered by intraperitoneal injection (10 mg/kg) daily and irradiation was delivered on the tumor-bearing leg for 15 Gy in 3 fractions. Surface markers of splenic neutrophils (G-MDSCs) and intratumoral neutrophils (tumor-associated neutrophils [TANs]) were assessed using flow cytometry and expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor 1 alpha (HIF-1α) of tumor was evaluated by immunohistochemical (IHC) staining. Results: The mean tumor volumes (±standard error) at the 15th day after randomization were 1,382.0 (±201.2) mm3 (group 1), 559.9 (±67.8) mm3 (group 3), and 370.5 (± 48.1) mm3 (group 4), respectively. For G-MDSCs, irradiation reversed decreased expression of CD101 from tumor-bearing mice, and additional increase of CD101 expression was induced by captopril administration. Similar tendency was observed in TANs. The expression of tumor-necrosis factor-associated molecules, CD120 and CD137, are increased by irradiation in both G-MDSCs and TANs. Further increment was observed by captopril except CD120 in TANs. For IHC staining, VEGF and HIF-1α positivity in tumor cells were decreased when treated with captopril. Conclusion: Captopril is suggested to have additional effect when combined to irradiation in a murine tumor model by modulation of MDSCs and angiogenesis. © 2016. The Korean Society for Radiation Oncology.
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Collections - Medicine > Department of Medicine > 1. Journal Articles
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