Antineuroinflammatory and Antiproliferative Activities of Constituents from Tilia amurensis
- Authors
- Kim, KH[Kim, Ki Hyun]; Moon, E[Moon, Eunjung]; Cha, SM[Cha, Joon Min]; Lee, S[Lee, Seulah]; Yu, JS[Yu, Jae Sik]; Kim, CS[Kim, Chung Sub]; Kim, SY[Kim, Sun Yeou]; Choi, SU[Choi, Sang Un]; Lee, KR[Lee, Kang Ro]
- Issue Date
- Oct-2015
- Publisher
- PHARMACEUTICAL SOC JAPAN
- Keywords
- Tilia amurensis; Tiliaceae; isoflavonoid glycoside; nitric oxide; antineuroinflammation; antiproliferation
- Citation
- CHEMICAL & PHARMACEUTICAL BULLETIN, v.63, no.10, pp.837 - 842
- Indexed
- SCIE
SCOPUS
- Journal Title
- CHEMICAL & PHARMACEUTICAL BULLETIN
- Volume
- 63
- Number
- 10
- Start Page
- 837
- End Page
- 842
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/42914
- ISSN
- 0009-2363
- Abstract
- As part of our ongoing search for bioactive constituents of natural Korean medicinal resources, we found in a preliminary study that the methanol (MeOH) extract from the trunks of Tilia amurensis RUPR. showed an inhibitory effect on nitric oxide (NO) production in an activated murine microglial cell line. A bioassay-guided fractionation and chemical investigation of the MeOH extract resulted in the isolation and identification of a new isoflavonoid glycoside, orobol 4'-O-beta-D-apiofuranosyl-(1-->6)-beta-D-glucopyranoside (1) and 16 known compounds (2-17). The structure of the new compound was determined by spectroscopic methods, i.e., one-dimensional (1D) and two-dimensional (2D)-NMR techniques and high resolution (HR)-MS, and chemical methods. The antineuroinflammatory activities of the isolated compounds were determined by measuring NO levels in the medium using murine microglial BV-2 cells. Among them, 12 compounds, including compound 1 (most active with an IC50 value of 23.42 mu M), inhibited NO production in lipopolysaccharide-stimulated BV-2 cells. Moreover, compounds 1-4 showed moderate antiproliferative activities against the SK-MEL-2 cell line, with IC50 values ranging from 12.31 to 19.67 mu M.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Pharmacy > Department of Pharmacy > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/42914)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.