A direct protein kinase B-targeted anti-inflammatory activity of cordycepin from artificially cultured fruit body of Cordyceps militaris
- Authors
- Yoon, JY[Yoon, Ju Young]; Kim, JH[Kim, Ji Hye]; Baek, KS[Baek, Kwang-Soo]; Kim, GS[Kim, Geum Soog]; Lee, SE[Lee, Seung Eun]; Lee, DY[Lee, Dae Young]; Choi, JH[Choi, Je Hun]; Kim, SY[Kim, Seung Yu]; Park, HB[Park, Hyun Bong]; Sung, GH[Sung, Gi-Ho]; Lee, KR[Lee, Kang Ro]; Cho, JY[Cho, Jae Youl]; Noh, HJ[Noh, Hyung Jun]
- Issue Date
- Jul-2015
- Publisher
- MEDKNOW PUBLICATIONS & MEDIA PVT LTD
- Keywords
- Anti-inflammatory activity; cordycepin; Cordyceps militaris (Clavicipitaceae); phytochemical study; protein kinase B
- Citation
- PHARMACOGNOSY MAGAZINE, v.11, no.43, pp.477 - 485
- Indexed
- SCIE
SCOPUS
- Journal Title
- PHARMACOGNOSY MAGAZINE
- Volume
- 11
- Number
- 43
- Start Page
- 477
- End Page
- 485
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/43555
- DOI
- 10.4103/0973-1296.60454
- ISSN
- 0973-1296
- Abstract
- Background: Cordyceps militaris is one of well-known medicinal mushrooms with anti-inflammatory, anti-cancer, anti-diabetic, and anti-obesity activities. Objective: The objective of the following study is to isolate chemical components from the ethanol extract (Cm-EE) from Cordyceps militaris and to evaluate their anti-inflammatory activities. Materials and Methods: Column chromatographic separation was performed and anti-inflammatory roles of these compounds were also examined by using NO production and protein kinase B (AKT) activity assays. Results: From Cm-EE, 13 constituents, including trehalose (1), cordycepin (2), 6-hydroxyethyladenosine (3), nicotinic amide (4), butyric acid (5), beta-dimorphecolic acid (6), alpha-dimorphecolic acid(7), palmitic acid (8), linoleic acid (9), cordycepeptide A (10), 4-(2-hydroxy-3-((9E,1 2E)-octadeca-9,12-dienoyloxy)propoxy)-2-(trimethylammonio)butanoate (11), 4-(2-hydroxy-3-(palmitoloxy)propoxy)-2-(trimethylammonio) butanoate (12), and linoleic acid methyl ester (13) were isolated. Of these components, compound 2 displayed a significant inhibitory effect on NO production in lipopolysaccharide (LPS)-activated RAW264.7 cells. Furthermore, this compound strongly and directly suppressed the kinase activity of AKT, an essential signalling enzyme in LPS-induced NO production,, by interacting with its ATP binding site. Conclusion: C. militaris could have anti-inflammatory activity mediated by cordycepin-induced suppression of AKT.
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- Appears in
Collections - Biotechnology and Bioengineering > Integrative Biotechnology > 1. Journal Articles
- Pharmacy > Department of Pharmacy > 1. Journal Articles
- Biotechnology and Bioengineering > Department of Genetic Engineering > 1. Journal Articles
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