Glucose-insulin-potassium solution protects ventricular myocytes of neonatal rat in an in vitro coverslip ischemia/reperfusion model
- Authors
- Chun W.-J.[Chun W.-J.]; Nah D.-Y.[Nah D.-Y.]; Bae J.-H.[Bae J.-H.]; Chung J.-W.[Chung J.-W.]; Lee H.[Lee H.]; Moon I.S.[Moon I.S.]
- Issue Date
- May-2015
- Publisher
- 대한심장학회
- Keywords
- Myocytes; cardiac; Glucose-insulin-potassium cardioplegic solution; GIcNAc; O-GIcNac transferase
- Citation
- Korean Circulation Journal, v.45, no.3, pp.234 - 241
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- Korean Circulation Journal
- Volume
- 45
- Number
- 3
- Start Page
- 234
- End Page
- 241
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/44028
- DOI
- 10.4070/kcj.2015.45.3.234
- ISSN
- 1738-5520
- Abstract
- Background and Objectives: The benefit of high glucose-insulin-potassium (GIK) solution in clinical applications is controversial. We established a neonatal rat ventricular myocyte (NRVM) in vitro coverslip ischemia/reperfusion (I/R) model and investigated the effects of GIK solution on suppressing reactive oxygen species (ROS) and upregulating O-GlcNacylation, which protects cells from ischemic injury. Materials and Methods: NRVMs were isolated from postnatal day 3-4 Sprague-Dawley rat pups and grown in Dulbecco's modified Eagle's medium containing high glucose (4.5 g/L), fetal bovine serum, and penicillin/streptomycin. The effects of the GIK solution on ROS production, apoptosis, and expression of O-GlcNAc and O-GlcNAc transferase (OGT) were investigated in the coverslip I/R model. Results: Covering the 24-well culture plates for 3 hr with 12 mm diameter coverslips resulted in the appropriate ischemic shock. Glucose and insulin synergistically reduced ROS production, protected NRVM dose-dependently from apoptosis, and altered O-GlcNAc and OGT expression. Conclusion: The high GIK solution protected NRVM from I/R injury in vitro by reducing ROS and altering O-GlcNacylation. Copyright © 2015 The Korean Society of Cardiology.
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Collections - Medicine > Department of Medicine > 1. Journal Articles
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