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Cited 6 time in webofscience Cited 5 time in scopus
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The Role of Binding Site on the Mechanical Unfolding Mechanism of Ubiquitinopen access

Authors
Cao, PH[Cao, Penghui]Yoon, G[Yoon, Gwonchan]Tao, WW[Tao, Weiwei]Eom, K[Eom, Kilho]Park, HS[Park, Harold S.]
Issue Date
4-Mar-2015
Publisher
NATURE PUBLISHING GROUP
Citation
SCIENTIFIC REPORTS, v.5
Indexed
SCIE
SCOPUS
Journal Title
SCIENTIFIC REPORTS
Volume
5
URI
https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/44312
DOI
10.1038/srep08757
ISSN
2045-2322
Abstract
We apply novel atomistic simulations based on potential energy surface exploration to investigate the constant force-induced unfolding of ubiquitin. At the experimentally-studied force clamping level of 100 pN, we find a new unfolding mechanism starting with the detachment between beta(5) and beta(3) involving the binding site of ubiquitin, the Ile44 residue. This new unfolding pathway leads to the discovery of new intermediate configurations, which correspond to the end-to-end extensions previously seen experimentally. More importantly, it demonstrates the novel finding that the binding site of ubiquitin can be responsible not only for its biological functions, but also its unfolding dynamics. We also report in contrast to previous single molecule constant force experiments that when the clamping force becomes smaller than about 300 pN, the number of intermediate configurations increases dramatically, where almost all unfolding events at 100 pN involve an intermediate configuration. By directly calculating the life times of the intermediate configurations from the height of the barriers that were crossed on the potential energy surface, we demonstrate that these intermediate states were likely not observed experimentally due to their lifetimes typically being about two orders of magnitude smaller than the experimental temporal resolution.
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