MiR-195a Inhibits Adipocyte Differentiation by Targeting the Preadipogenic Determinator Zfp423
- Authors
- Yun U.J.[Yun U.J.]; Song N.-J.[Song N.-J.]; Yang D.K.[Yang D.K.]; Kwon S.-M.[Kwon S.-M.]; Kim K.[Kim K.]; Kim S.[Kim S.]; Jo D.-G.[Jo D.-G.]; Park W.J.[Park W.J.]; Park K.W.[Park K.W.]; Kang H.[Kang H.]
- Issue Date
- 2015
- Citation
- Journal of Cellular Biochemistry, v.116, no.11, pp.2589 - 2597
- Journal Title
- Journal of Cellular Biochemistry
- Volume
- 116
- Number
- 11
- Start Page
- 2589
- End Page
- 2597
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/49117
- DOI
- 10.1002/jcb.25204
- Abstract
- MicroRNAs (miRNAs) play essential roles in various cellular processes including proliferation and differentiation. In this study, we identified miRNA-195a (miR-195a) as a regulator of adipocyte differentiation. Differential expression of miR-195a in preadipocytes and adipocytes suggests its role in lipid accumulation and adipocyte differentiation. Forced expression of miR-195a mimics suppressed lipid accumulation and inhibited expression of adipocyte markers such as PPARγ and aP2 in 3T3-L1 and C3H10T1/2 cells. Conversely, downregulation of miR-195a by anti-miR-195a increased lipid accumulation and expression of adipocyte markers. Target prediction analysis suggested zinc finger protein 423 (Zfp423), a preadipogenic determinator, as a potential gene recognized by miR-195a. In line with this, mimicked expression of miR-195a reduced the expression of Zfp423, whereas anti-miR-195a increased its expression. Predicted targeting sequences in Zfp423 3'UTR, but not mutated sequences fused to luciferase, were regulated by miR-195a. Ectopic Zfp423 expression in 3T3-L1 cells increased lipid accumulation and expression of adipocyte markers, consistent with the observation that miR-195a targets Zfp423, resulting in suppressed adipocyte differentiation. In addition, miR-195a and Zfp423 were inversely correlated in obese fat tissues, raising the possibility of miRNA's role in obesity. Together, our data show that miR-195a is an anti-adipogenic regulator, which acts by targeting Zfp423, and further suggest the roles of miR-195a in obesity and metabolic diseases. J. Cell. Biochem. 116: 2589-2597, 2015. © 2015 Wiley Periodicals, Inc.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Pharmacy > Department of Pharmacy > 1. Journal Articles
- Biotechnology and Bioengineering > Department of Food Science and Biotechnology > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/49117)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.