Cytochalasin B Modulates Macrophage-Mediated Inflammatory Responses
- Authors
- Kim, MY[Kim, Mi-Yeon]; Kim, JH[Kim, Jong-Hoon]; Cho, JY[Cho, Jae Youl]
- Issue Date
- 31-Jul-2014
- Publisher
- KOREAN SOC APPLIED PHARMACOLOGY
- Keywords
- Actin cytoskeleton; Inflammation; Cytochalasin B; Macrophages; TLR4
- Citation
- BIOMOLECULES & THERAPEUTICS, v.22, no.4, pp.295 - 300
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BIOMOLECULES & THERAPEUTICS
- Volume
- 22
- Number
- 4
- Start Page
- 295
- End Page
- 300
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/52272
- DOI
- 10.4062/biomolther.2014.055
- ISSN
- 1976-9148
- Abstract
- The actin cytoskeleton plays an important role in macrophage-mediated inflammatory responses by modulating the activation of Src and subsequently inducing nuclear factor (NF)-kappa B translocation. In spite of its critical functions, few papers have examined how the actin cytoskeleton can be regulated by the activation of toll-like receptor (TLR). Therefore, in this study, we further characterized the biological value of the actin cytoskeleton in the functional activation of macrophages using an actin cytoskeleton disruptor, cytochalasin B (Cyto B), and explored the actin cytoskeleton's involvement in morphological changes, cellular attachment, and signaling events. Cyto B strongly suppressed the TLR4-mediated mRNA expression of inflammatory genes such as cyclooxygenase (COX)-2, tumor necrosis factor (TNF)-alpha, and inducible nitric oxide (iNOS), without altering cell viability. This compound also strongly suppressed the morphological changes induced by lipopolysaccharide (LPS), a TLR4 ligand. Cyto B also remarkably suppressed NO production under non-adherent conditions but not in an adherent environment. Cyto B did not block the co-localization between surface glycoprotein myeloid differentiation protein-2 (MD2), a LPS signaling glycoprotein, and the actin cytoskeleton under LPS conditions. Interestingly, Cyto B and PP2, a Src inhibitor, enhanced the phagocytic uptake of fluorescein isothiocyanate (FITC)-dextran. Finally, it was found that Cyto B blocked the phosphorylation of vasodilator-stimulated phosphoprotein (VASP) at 1 min and the phosphorylation of heat shock protein 27 (HSP27) at 5 min. Therefore, our data suggest that the actin cytoskeleton may be one of the key components involved in the control of TLR4-mediated inflammatory responses in macrophages.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Biotechnology and Bioengineering > Integrative Biotechnology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.