Comparison of the Digene HPV Genotyping LQ Test and the PANArray HPV Genotyping Chip for Detection of High-Risk or Probable High-Risk Human Papillomavirus Genotypes
- Authors
- Park, KS[Park, Kyung Sun]; Kim, JY[Kim, Ji-Youn]; Ki, CS[Ki, Chang-Seok]; Lee, NY[Lee, Nam Yong]
- Issue Date
- Jul-2014
- Publisher
- KOREAN SOC LABORATORY MEDICINE
- Keywords
- Human papillomavirus; DNA test; Comparison; Genotyping
- Citation
- ANNALS OF LABORATORY MEDICINE, v.34, no.4, pp.279 - 285
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- ANNALS OF LABORATORY MEDICINE
- Volume
- 34
- Number
- 4
- Start Page
- 279
- End Page
- 285
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/52481
- DOI
- 10.3343/alm.2014.34.4.279
- ISSN
- 2234-3806
- Abstract
- Background: We evaluated the performance of two different array-based techniques, a bead-based multiplex genotyping method (LQ; digene HPV Genotyping LQ Test, QIAGEN, Germany) and a DNA chip-based method using peptide nucleic acid probes (PANArray; PANArray HPV Genotyping Chip, Panagene, Korea), for detection of human papillomavirus (HPV) and genotyping of high-risk (HR) or probable high-risk (PHR) HPVs in healthy patients who visited a health-promotion center. Methods: We obtained 508 unselected, consecutive cervicovaginal swab specimens. All specimens were examined by using the PANArray and LQ tests. All HPV-positive samples were then analyzed by multiplex PCR and direct sequencing. Results: The LQ test detected 47 HPV-positive cases (9.3%) with HR or PHR genotypes and the PANArray test identified 36 cases (7.1%). When the results of LQ and PANArray were compared by using comprehensive genotyping (integrated interpretation of the results of LQ, PANArray, multiplex PCR, and direct sequencing) for the detection of HR or PHR genotypes, the kappa values were 0.44 and 0.30 for LQ and PANArray, respectively. In comparison to comprehensive genotyping, the LQ test yielded 53 (60.0%) concordant and 12 (13.5%) compatible results, and the PANArray yielded 36 (40.4%) concordant and three (3.4%) compatible results. Conclusions: The results of the LQ test had higher concordance and/or greater compatibility with those of comprehensive genotyping for the detection of HR or PHR genotypes than those of the PANArray test.
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Collections - Medicine > Department of Medicine > 1. Journal Articles
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