alpha 2-Adrenergic agonists including xylazine and dexmedetomidine inhibit norepinephrine transporter function in SK-N-SH cells
- Authors
- Park, JW[Park, Jin Won]; Chung, HW[Chung, Hyun Woo]; Lee, EJ[Lee, Eun Jeong]; Jung, KH[Jung, Kyung-Ho]; Paik, JY[Paik, Jin-Young]; Lee, KH[Lee, Kyung-Han]
- Issue Date
- 29-Apr-2013
- Publisher
- ELSEVIER IRELAND LTD
- Keywords
- alpha 2 adrenergic agonist; Nisoxetine; Dexmedetom
- Citation
- NEUROSCIENCE LETTERS, v.541, pp.184 - 189
- Indexed
- SCIE
SCOPUS
- Journal Title
- NEUROSCIENCE LETTERS
- Volume
- 541
- Start Page
- 184
- End Page
- 189
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/60860
- DOI
- 10.1016/j.neulet.2013.02.022
- ISSN
- 0304-3940
- Abstract
- alpha(2)-Adrenergic agonists simulate norepinephrine (NE) action on alpha(2) receptors of sympathetic neurons to mediate feedback inhibition of NE release. These agents are used as valuable adjuncts for management of hypertension and for anesthesia. Their action, equivalent to NE on alpha 2 adrenergic receptors, raises the question whether alpha 2 agonists may also target NE transporters (NETs), another major control mechanism for noradrenergic neurotransmission. We thus investigated the effect of alpha(2) agonists on transport of the NE analog, I-131-metaiodobenzylguanidine (MIBG). Results from this investigation showed that xylazine and dexmedetomidine dose-dependently blocked [H-3]nisoxetine binding in neuron-like SK-N-SH cells. Furthermore, the agents acutely suppressed cellular MIBG uptake in a dose-dependent manner. This effect was uninfluenced by the alpha 2 antagonist yohimbine, but was completely reversed by drug removal. There was no change in membrane NET density by the agents. Moreover, saturation analysis showed that xylazine and dexmedetomidine significantly increased Km without affecting V-max, indicating competitive inhibition of MIBG transport. Thus, the alpha(2) adrenergic agonists xylazine and dexmedetomidine, acutely suppress NET function through competitive inhibition of substrate transport, likely by direct interaction on a region that over-laps with the nisoxetine binding site. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
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Collections - Medicine > Department of Medicine > 1. Journal Articles
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