Gel electrophoretic mobility evaluation of a necklace-like DNA nanostructure
- Authors
- Um, SH[Um, Soong Ho]
- Issue Date
- Feb-2013
- Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
- Keywords
- X-shaped DNA; ligation; necklace nanostructure
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.18, no.1, pp.218 - 221
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
- Volume
- 18
- Number
- 1
- Start Page
- 218
- End Page
- 221
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/61588
- DOI
- 10.1007/s12257-012-0472-9
- ISSN
- 1226-8372
- Abstract
- DNA nanotechnologies have been highlighted as a promising synthetic tool for the creation of new shaped materials. They have developed a variety of materials in different shapes and sizes [1]. Inspired by these advancements, we sought to design a ring-shaped DNA nanostructure connected by X-DNA blocks. Six XDNA blocks were ligated together to form a circular nanostructure with a diameter of approximately 30 nm. Each DNA block possesses different overhang sequences in its terminal. It was sequentially built up onto each block platform in the line and later clipped into a necklace shape via enzymatic ligation. It was finally evaluated by a gel electrophoretic migration shift assay. It was concluded that the complete set of the necklace shaped DNA nanostructure was the most slowly retarded relative to other forms of incompleteness.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Engineering > School of Chemical Engineering > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.