The Rhizomes of Acorus gramineus and the Constituents Inhibit Allergic Response In vitro and In vivo
- Authors
- Lim, H[Lim, Hyun]; Lee, SY[Lee, Seung Young]; Lee, KR[Lee, Kang Ro]; Kim, YS[Kim, Yeong Shik]; Kim, HP[Kim, Hyun Pyo]
- Issue Date
- 30-Sep-2012
- Publisher
- KOREAN SOC APPLIED PHARMACOLOGY
- Keywords
- Acorus gramineus; beta-Asarone; 2,3-Dihydro-4,5,7-
- Citation
- BIOMOLECULES & THERAPEUTICS, v.20, no.5, pp.477 - 481
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BIOMOLECULES & THERAPEUTICS
- Volume
- 20
- Number
- 5
- Start Page
- 477
- End Page
- 481
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/64214
- DOI
- 10.4062/biomolther.2012.20.5.477
- ISSN
- 1976-9148
- Abstract
- The rhizomes of Acorus gramineus have frequently been used in traditional medicine mainly for sedation as well as enhancing brain function. In this study, the anti-allergic activity of A. gramineus was investigated. The 70% ethanol extract of the rhizomes of A. gramineus was found to inhibit the allergic response against 5-lipoxygenase (5-LOX)-catalyzed leukotriene (LT) production from rat basophilic leukemia (RBL)-1 cells and beta-hexosaminidase release from RBL-2H3 cells with IC50's of 48.9 and >200 mu g/ml, respectively. Among the 9 major constituents isolated, beta-asarone, (2R,3R,4S,5S)-2,4-dimethyl-1,3-bis (2',4',5'-trimethoxyphenyl) tetrahydrofuran (AF) and 2,3-dihydro-4,5,7-trimethoxy-1-ethyl-2-methyl-3-(2,4,5-trimethoxyphenyl)indene (Al) strongly inhibited 5-LOX-catalyzed LT production in A23187-treated RBL-1 cells, Al being the most potent (IC50=6.7 mu M). Against beta-hexosaminidase release by antigen-stimulated RBL-2H3 cells, only Al exhibited strong inhibition (IC50=7.3 mu M) while beta-asarone and AF showed 26.0% and 39.9% inhibition at 50 mu M, respectively. In addition, the ethanol extract of A. gramineus showed significant inhibitory action against the hapten-induced delayed hypersensitivity reaction in mice by oral administration at 200 mg/kg. Therefore, it is suggested that A. gramineus possesses anti-allergic activity and the constituents including beta-asarone and Al certainly contribute to the anti-allergic activity of the rhizomes of A. gramineus.
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Collections - Pharmacy > Department of Pharmacy > 1. Journal Articles
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